Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis -- Sasakura et al. 102 (42): 15134 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Sasakura et al. 10.1073/pnas.0503640102.

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Supporting Figure 4
Supporting Table 4

Supporting Figure 4

Fig. 4. (A) Apoptosis in the tail of a swimming juvenile mutant larva (sj) and a normal larva. Clear TUNEL signals were observed in the nuclei of cells in the tail of both larvae (arrows). (Bar, 100 mm.) (B) BrdUrd incorporation (green signals) in sj mutant larvae at 3 days postfertilization (dpf) was significantly decreased compared with a normal larva and juvenile. (Bar, 100 mm.)

Table 4. The efficiency of attachment of sj larvae

Experiment number	Number of swimming larvae	Number of attached juveniles	Number of metamorphosed but not attached juveniles	Number of omitted or lost individuals*
Exp.1-1dpf-wt	40	16	2	42
Exp.2-1dpf-wt	36	31	2	31
Exp.1-1dpf- sj	86	1	5	8
Exp.2-1dpf- sj	91	1	1	7

One hundred larvae per experiment were isolated in a Petri dish at 1 dpf and cultured for 2 days. After 2 days, the number of swimming (unattached) larvae, attached and metamorphosed juveniles, and metamorphosed but not attached juveniles was scored. The number of attached juveniles was reduced dramatically in sj mutants.

*Larvae entrapped at the surface of the seawater were omitted, because most of them died. Normal larvae swam more vigorously than sj larvae and entrapped at the surface more frequently.