Supporting information for Dudley et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.152333499

 

Table 3. Mutations in sequences from downstream of S

m in WT and AID–/–-derived hybridomas

Sm rearranged

S

m nonrearranged

Hybridoma

# Alleles

Mutations*

Hybridoma

# Alleles

Mutations

WT hybridomas

W1

2

1/0

C3

2

0/0

W2

2

3/0

C4

2

0/0

W3

1

1

C5

2

1/0

W4

2

1/2

C6

2

1/0

W5

2

0/0

C7

2

0/0

W7

2

0/0

C8

2

0/0

W10

2

0/0

  

   

   

W11

2

1/0

  

  

  

W12

2

2/0

   

  

  

AID-deficient

A1

2

0/0

AC1

2

0/0

A2

2

0/0

AC2

2

0/0

A3

2

0/0

AC3

2

0/0

A4

2

0/0

   

  

  

A5

2

0/0

   

  

  

W, WT B cell-derived hybridoma with rearrangements detected in S

m (see Table 2 for details). C, WT B cell-derived hybridoma with no detectable Sm rearrangements. A, AID-/--derived B cell hybridoma with rearrangements detected in Sm (see Table 2 for details). AC, AID-/- B cell-derived hybridoma with no detectable Sm rearrangements.

*

The number of bp changes detected on each allele in the 300-bp region downstream of Sm, as shown in Fig. 3.