Blumenstiel and Hartl. 10.1073/pnas.0508192102. |
Fig. 5. XˆX and X9 crosses and backcrosses (BC). Dys, dysgenic.
Fig. 6. Cross schemes for lances A, B, C, and D of Fig. 3. Cytoplasm inherited from the mother is designated black if the mother possessed any chromosomes from strain 160. Lack of the X chromosome from 160 in such a case is specified.
Fig. 7. XˆX/9 and nondysgenic (NDS) crosses to determine whether repression of male dysgenesis was a true maternal effect.
Fig. 8. Crosses used to measure the maternal effect of strain 160 chromosomes. (A) Crossing scheme to determine the strength of each of the chromosomes from strain 160 as a maternal suppressor of dysgenesis. (B) The number of crosses and number of male flies counted for each maternal genotype. (C) The proportion of testes that were atrophied in the males of each cross as a function of the number of maternal chromosomes from strain 160. We excluded the sixth chromosome because it had only a negligible effect as a suppressor. Dys, dysgenic; Ndys, nondysgenic.
Fig. 9. Of the nine components of the RNA interference machinery that are significantly different in expression between ovary and testes in Drosophila melanogaster, seven are up-regulated in ovaries. Gene expression data were taken from ref. 1 and analyzed using Bayesian Analysis of Gene Expression Levels (2). Significance of difference in gene expression was determined based on whether 95% credible intervals for relative gene expression overlapped.
1. Parisi, M., Nuttall, R., Naiman, D., Bouffard, G., Malley, J., Andrews, J., Eastman, S. & Oliver, B. (2003) Science 299, 697–700.
2. Townsend, J. P. & Hartl, D. L. (2002) Genome Biol. 3, research0071.1–research0071.16.