Supporting Information

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Fig. 4. Electron micrographs of AmyBergerac (AMYBG) (pH 2.6, c » 70 mM, and t = 3 months). (a) Incubation at room temperature. (b) Incubation at 37°C. The black arrows point to some of the numerous nuclei that can be found in this sample. Figure corresponds to Stability of a–SH3 Proteins with Amyloidogenic Insertions.

Fig. 5. Binding of thioflavin (ThT) to mature fibrils of 1-SH. Figure corresponds "Conformational Transition and Amyloid Fibril Formation of a–SH3 Variants with Amyloidogenic Insertions.

Fig. 6. Far-UV (Left) and near-UV (Right) evolution of all a-SH3 variants studied in this work (t = 0 and 3 months). Electron micrographs of the C-terminus variants are also shown (magnification: 89,000). Figure corresponds to Conformational Transition and Amyloid Fibril Formation of a–SH3 Variants with Amyloidogenic Insertion.

Fig. 7. CD (a) and EM (b) analysis of the insoluble (b1) and soluble (b2) fractions of Ab-SH samples (pH 2.6, c » 300 mM, t = 8 months, and room temperature). Figure corresponds Conformational Transition and Amyloid Fibril Formation of a–SH3 Variants with Amyloidogenic Insertions.

Fig. 8. Far-UV CD of a 300-mM solution of 1-SH incubated for 18 months at pH 2.6 and room temperature. Figure corresponds to Conformational Transition and Amyloid Fibril Formation of a–SH3 Variants with Amyloidogenic Insertions.

Fig. 9. Effect of phosphate salts (between 1 and10 mM) in 1-SH amyloid fibril formation. Far-UV CD and electron micrograph of a 300-mM sample incubated for 6 days at pH 2.6 and room temperature.Figure corresponds to Conformational Transition and Amyloid Fibril Formation of a–SH3 Variants with Amyloidogenic Insertions.

Fig. 10. Isolation of the protease-resistant core of mature fibrils. (a) Extensive pepsin proteolysis of 1-SH mature fibrils isolated by ultracentrifugation. (b) MS characterization of the insoluble fraction of the proteolysis reaction of 1-SH and result of the sequencing. Figure corresponds to Isolation of the Protease-Resistant Core of 1-SH Fibrils.

Fig. 11. Aminopeptidase resistance of 1-SH mature fibrils. The peaks corresponding to the reaction products are labeled with the name of the species. Figure corresponds to Isolation of the Protease-Resistant Core of 1-SH Fibrils.