Genomic expression programs and the integration of the CD28 costimulatory signal in T cell activation -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Diehn et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.092284399

Supporting Figure 8
Fig. 8.
GSK3 inhibition by lithium induces IL-2 production by T cells treated with aCD3 beads. T cells were isolated as described in Materials and Methods and cultured at 2 ´ 10⁶/ml. Indicated samples were pretreated with 10 mM lithium or 24 nM FK506 for 15 min. Cells were then mock-stimulated or stimulated with aCD3 or aCD3/aCD28 beads for 6 or 12 h. The concentration of IL-2 in the supernatant was measured by using a luminescence-based IL-2 ELISA (R & D Systems).