Supporting information for Pavlov et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.202127199
Fig. 6.
(A) Dependencies of initial rates for primer extension reaction on substrate concentration. The initial rates of reactions catalyzed by Taq DNA polymerase (·), TopoTaq in the presence of 0.2 M NaCl (o), and TopoTaq in the presence of 0.2 M NaCl and1M betaine (D) are plotted vs. concentration of PTJ. (B) Dependencies of initial rates of the primer extension reaction ([PTJ] = 0.72 µM) on concentration of TopoTaq with 0.3 M NaCl (o) and with 0.3 M NaCl and1M betaine (D) added to the reaction. The rate for 10.5 µg/ml TopoTaq without additions is shown for comparison (·).