Supporting information for Gauthier et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.202319099
Table 1. Binding analysis of recombinant proteins on different cell lines, measured by flow cytometry
Cell line | Recombinant proteins | ||||
Fab-like 1E8 | Fab-like Nalm6 | scSLC | Fab 1E8 | ||
Human adherent cell lines | |||||
BM stroma | C6 | + | + | + | – |
C8 | + | + | + | – | |
Fibroblast | MRC5 | + | + | + | – |
Epithelium | Hela | – | – | – | – |
Human lymphoid cell lines | |||||
Pro-B | Jea2 | – | – | – | – |
Reh | – | – | – | nd | |
Rs4.11 | – | – | – | nd | |
Pre-B | Nalm6 | – | – | – | – |
Laz221 | – | – | – | nd | |
Pre-B/B | 1E8 | – | – | – | nd |
B | Namalwa | – | – | – | nd |
Ramos | – | – | – | nd | |
Daudi | – | – | – | nd | |
C1R | – | – | – | nd | |
T | Jurkat | – | – | – | nd |
HSB2 | – | – | – | nd | |
Human myeloid cell line | |||||
U937 | – | – | – | nd | |
Murine adherent cell lines | |||||
BM stroma | MS5.1 | + | + | + | – |
ST2 | nd | nd | + | nd | |
Thymic stroma | MTE 1p | – | – | – | nd |
MTE 4.14 | – | – | – | nd | |
Endothelium | E-end | – | – | – | nd |
Murine lymphoid cell lines | |||||
Pro-B | 38B9 | – | – | – | nd |
Pre-B | 70Z3 | – | – | – | nd |
Myeloma | X63 | – | – | – | nd |
Simian adherent cell line | |||||
Epithelium | Cos 7 | + | + | + | nd |
Fab-like 1E8, Fab-like Nalm6, scSLC, and Fab 1E8 were produced in baculovirus system.
+, positive staining; –, no detectable staining; nd, not done.