Supporting information for Paszkowski et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.202474599

 

Table 1. Sequences of forward and reverse primers and 6-FAM 5

' end-labeled probes designed for the 3' UTR of the rice Pi transporter genes and the rice polyubiquitin 1 (RubQ1) gene for quantitative RT-PCR

Forward primer

Reverse primer

Probe

OsPT1

CGCTTCCGTACGAGTGGTAGT

GGTTCTTTCAAATCCAGGGAAA

CACCAATTCTTCAAGAACATATACACACAT

OsPT2

GACGAGACCGCCCAAGAAG

TTTTCAGTCACTCACGTCGAGAC

GATCGCCCAAGCGTGATGTCATAAACAT

OsPT3

TGCGACTGCTGATTCAGTACGT

ACAAATGCCATCAAATATGAACAGA

CATTTGGACACTGCCATTCACATCACACT

OsPT4

TTCTGCTAGTGTACCAAACAAAATTACA

CTAAGTGGCATTTATAATATCAACAGTAACC

TACTAGTCGTCAACCCAGGCAACGCA

OsPT5

TGCTACTGCCCATGACTAGGATT

CCATAGAAGAGATCCAGAGAAGCTGTA

CAACATGGTGAAAATCTGCTGGTTCCCA

OsPT6

CCGCCCCTGCAAACTGTA

CAACTGGCGGTTTCTTCGAT

TAGCGCTGTCGCCGTCTGC

OsPT7

GCAAGTCGCTCGAGGAGATG

TGGAATTAACGGGTGGATCAC

CTGGCCGACCTCGTTCTCCCGT

OsPT8

AGAAGGCAAAAGAAATGTGTGTTAAAT

AAAATGTATTCGTGCCAAATTGCT

TCTGCAAGAATTATTCCTCAAGTGGCCAAA

OsPT9

AGAAAAACATAGGCTTGTCATCCTTT

AAAACCTAAGAAGCACTGTAAATAAATCC

CGTTTCCGCTCCTCCTGCCAATC

OsPT10

ATGTCGCCCATCCTTCCA

TCGCTTTCCGACGATGATC

AGTTCACTCACACGGAGACCCGGG

OsPT11

GAGAAGTTCCCTGCTTCAAGCA

TGCATATCCCAGATGAGCGTATC

CGAATGGCAGACATCCATGCACG

OsPT12

AACGAGATGACGAACACTTGCA

TCCTGTACCTAAAAAGCAAGTACTAACATAGT

TTCCTATCACGCTACAAGTACGAGCTTCGC

OsPT13

TTAGCATAATTCTTTTAGTAGTTAAATAGGAGATG

TGATTTAAGATAAGGATTGAATGCACAT

TTGGTGGCAGATTTCTATCCGTGACACTC

RubQ1

GGGTTCACAAGTCTGCCTATTTG

ACGGGACACGACCAAGGA

CAGACCAGACAACCATAGCTCCATTGGG