Supporting Figures

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Fig. 5. Characterization of Cyth25 undifferentiated cells. (a) EGFP+ hESC colonies were positive for the undifferentiated markers TRA-1-81, SSEA-4, and TRA-1-60 but negative for the murine ES cell marker SSEA-1. Images were taken from the colonies’ borders to use the murine feeder layer cells as an internal control. (b) Undifferentiated Cyth25 hESC colonies were positive for hNA. Note that the hNA antibody did not strongly react with the murine feeder layer. (c) Morphological aspects of two hESC undifferentiated colonies (colonies’ centers are marked by asterisks) chosen for transplantation. (Scale bar, 50 mm.)

Fig. 6. Examples of poor (Left) or no (Right) integration of transplanted cells. EGFP+ hESC that did not migrate from ventricles remained attached to the ependymal layer as clusters. ve, ventricles. (Scale bar, 50 mm.)

Fig. 7. Similar morphometric dimensions of hESC-derived, calbindin-expressing neurons in mouse hippocampus. EGFP+ neurons have similar diameters when compared with host cells in the same region. (Scale bar, 10 mm.)