Brain estrogen deficiency accelerates A{beta} plaque formation in an Alzheimer's disease animal model -- Yue et al. 102 (52): 19198 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Yue et al. 10.1073/pnas.0505203102.

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Supporting Figure 5

Fig. 5. Effects of estrogen depletion on Ab clearance and NEP- and IDE-mediated Ab degradation. (A) Sagittal brain slides from 12-month-old APP23/Ar^+/- animals were immunostained with anti-Ab and CD11 and showed colocalization of activated microglia and plaques. Microglia were isolated from WT and Ar^+/- mice at postnatal 0-3 days and incubated with fluorescent-labeled Ab (100 nM) for 24 h with 24-h pretreatment of 17b-estradiol (100 nM) in combination with ICI 182,789 or vehicle. (B Upper) A decrease in Ab uptake was observed in the Ar^+/--derived microglia compared with the microglia from WT mice at 6- and 24-h incubation. (B Lower) Estrogen treatment reversed the impairment of Ab uptake in Ar^+/--derived microglia with partial dependency on estrogen receptors. (C and D) IDE and NEP protein expression in 6- and 12-month-old WT (n = 10), APP23 (n = 13), and APP23/Ar^+/- (n = 12) mice. *, P < 0.05; **, P < 0.01 (compared with WT). #, P < 0.05 (compared with APP23 mice).