Table S18 - RMPchr2

Table S18: Tiling path of RMP contigs combined to form cRMP chromosome 2.
Tiling paths of P. berghei (Pb), P. c. chabaudi (Pc) and P. y. yoelii (Py) contigs combined into composite rodent malaria parasite (cRMP)
contigs aligned against parts of the 14 chromosomes of P. falciparum (Pf). The cRMP contigs are further linked by physical linkage
and PCR (see Methods) to form cRMP scaffolds. Different synteny blocks with homology to different P. falciparum chromosomes
together form the cRMP chromosomes. The tables also show gaps in the alignment (black lines), cRMP telomeres (white bars) and
predicted centromeres (plum/black bars). Contigs, physical groups or PCR groups interrupted by inter- or intrasyntenic regions are
printed in bold. A summary of the number of RMP contigs, cRMP contigs and cRMP scaffolds as well as their average, minimum,
maximum and total sizes is provided. Column headings are as follows:
Pf chr.: P. falciparum chromosome to which the RMP contigs are aligned
(#: PROmer analysis  from this study; JC# and TK#: Carlton et al.; man#: manual BLAST analysis;
i: inversed orientation of the synteny block; colours refer to those used in Fig. 2 of the paper);
Pb Pc Py contig: names of the aligned RMP contigs;
Size: size (bp) of the aligned RMP contigs;
+/-: orientation of the aligned RMP contigs;
Start contig & End contig: estimated start & end coordinates of alignment of the RMP contigs based on the coordinates
of PROmer hits corrected for the contig’s size and orientation and presence of inter- and intrasyntenic regions
(the start position of the first RMP contig in the alignment was set at zero);
cRMP contig: names of cRMP contigs
(the first 2 digits refer to the cRMP chromosome number and the following 3 digits designate the order of the contigs);
cRMP contig size: size of cRMP contigs based on the corrected coordinates of alignment;
Gap size to next cRMP contig: size of gaps between neighbouring cRMP contigs;
Start cRMP contig & End cRMP contig: estimated start & end coordinates of alignment of the cRMP contigs;
Phys. group (Py): physical linkage of neighbouring P. y. yoelii contigs;
PCR group (Py): neighbouring P. y. yoelii contigs were linked previously or for this study by PCR;
PCR size (Py): size of the PCR product;
cRMP scaffold: names of cRMP scaffolds
(the first 2 digits refer to the cRMP chromosome number and the following 2 digits designate the order of the scaffolds);
cRMP scaffold size: size of cRMP scaffolds;
Gap size to next cRMP scaffold: size of gaps between neighbouring cRMP scaffolds;
Start cRMP scaffold & End cRMP scaffold: estimated start & end coordinates of alignment of the cRMP scaffolds;
Overlap: Linkage between RMP contigs established through manual BLAST analysis.
Pf chr. Pb Pc Py contig Size +/- Start contig End contig cRMP contig cRMP contig size Gap size to next cRMP contig Start cRMP contig End cRMP contig Phys. group (Py) PCR group (Py) PCR size (Py) cRMP scaffold cRMP scaffold size Gap size to next cRMP scaffold Start cRMP scaffold End cRMP scaffold Overlap (manual)
Telomere                                    
Centromere                                    
i1 MALPY00384 4825 + 0 4946 1239A 256 450
i1 Pb.c000501475.Contig1 3775 - 2567 6834    
i1 Pc.c001605839.Contig1 999 + 3510 4527    
i1 Pc.c001606333.Contig1 1347 + 4972 6319    
i1 MALPY02016 3722 - 5342 9064 cRMPc02001 9064 1759 0 9064 1239A 256 500
i1 MALPY02965 2044 + 10823 12870 cRMPc02002 2047 458 10823 12870 10 256 3500
i1 Pb.c001803349.Contig1 9349 + 13328 22711    
i1 MALPY01800 7280 + 13395 20568 10 22 600
i1 Pc.c000702653.Contig1 3306 - 14211 17658    
i1 MALPY03449 487 + 17401 17888   256
i1 Pc.c000703844.Contig1 3655 - 17889 22341    
i1 MALPY01329 6532 - 20359 26980 10 22 3000
i1 Pb.c001803514.Contig1 3015 + 22930 26100 cRMPc02003 13652 1606 13328 26980    
i1 MALPY00652 5844 - 28586 34526 10 27 600
i1 PbContig356 8269 - 28794 38356    
i1 Pc.c000402888.Contig1 3786 - 30429 34329    
i1 MALPY03422 583 + 31063 31655   22
i1 MALPY02664 2037 - 34975 37422 10 27
i1 PcContig144 1952 - 35107 37508  
i1 MALPY02210 4396 - 35781 40251 10 65 6500
i1 PbContig191 9601 - 38837 50841  
i1 MALPY00660 10299 - 40009 52318 149  
i1 PcContig498 10371 + 40310 52926    
i1 MALPY03056 536 + 49613 50149 cRMPc02004 24340 2478 28586 52926   65
i1 MALPY00118 18636 - 55404 74438 149
i1 PbContig177 11746 - 59101 71697
i1 Pc.c000903874.Contig1 1214 + 60441 61740
i1 PcContig276 10052 + 62367 72502
i1 Pb.c001301503.Contig1 5480 - 72144 77526
i1 Pc.c001103706.Contig1 4477 + 73644 78081
i1 MALPY03410 1020 + 74481 75513 230 1800
i1 MALPY00378 4550 + 76475 81071 61 230
i1 PcContig754 1702 + 78400 80151  
i1 Pb.c001202028.Contig1 1628 - 78443 80122 cRMPc02005 25667 311 55404 81071  
i1 MALPY00870 3360 + 81382 84745 cRMPc02006 3363 571 81382 84745 61 63 1200
i1 Pb.c000701602.Contig1 3639 - 85316 88931    
i1 Pc.c001103685.Contig1 3792 - 85487 89582    
i1 MALPY02998 1997 + 85722 87695 61 29 1100
i1 MALPY03011 1602 + 86381 87983 61 63
i1 MALPY02346 3248 + 88640 91740 75 29 500
i1 Pb.c000001279.Contig1 7992 - 89351 98690    
i1 Pc.c001104242.Contig1 933 + 89652 90556    
i1 Pc.c001706619.Contig1 1977 + 90985 92974    
i1 MALPY03455 886 - 91990 92882   29
i1 MALPY01593 4320 + 92046 97504 75
iTK1 MALPY01511 4844 + 98271 103055 cRMPc02007 17739 1606 85316 103055 75
i1 MALPY00171 15668 - 104661 120416 75 54 2300
i1 Pc.c000702695.Contig2 10109 - 113579 125466  
i1 Pb.c001301843.Contig1 1784 - 116409 118343  
i1 MALPY03254 668 + 116813 117552 221 750
i1 MALPY03488 737 - 117638 118318 221
i1 Pb.c001301842.Contig1 1546 - 118630 120176  
i1 MALPY02068 10689 + 124149 136110 554 54 1100
i1 Pb.c000901449.Contig2 4079 + 125767 130674    
i1 Pc.c001003239.Contig1 2638 - 125897 128575    
i1 Pc.c001606491.Contig1 1081 - 129091 130427    
i1 Pc.c000202720.Contig1 3485 - 130783 134672    
i1 PbContig311 6560 + 133514 139234    
i1 MALPY02342 3881 + 135815 138919 554 24 9000
i1 PcContig71 5354 - 136221 140783  
i1 MALPY03063 772 + 136816 137588 cRMPc02008 36122 4369 104661 140783 54
i1 MALPY01184 7418 + 145152 152866 384A 24 350
i1 PbContig305 3201 - 146422 149671    
i1 Pc.c001002744.Contig1 3832 + 147471 151714    
i1 Pb.c000301473.Contig1 2683 - 149912 152889    
i1 Pc.c000202874.Contig1 1933 + 152070 152070    
iman1 MALPY02252 - Ins 4181 - 152687 157015 384A 24 600
i1 Pc.c000103062.Contig1 1015 - 154684 155699 cRMPc02009 11863 79 145152 157015    
i1 MALPY02524 6344 + 157094 163291 384A 24 400
i1 Pc.c001405315.Contig1 2467 - 157915 160396  
i1 Pc.c001203859.Contig1 3032 - 161478 164519  
i1 PbContig354 7851 + 162134 162134  
i1 Pb.c000101374.Contig1 4940 + 162830 167867  
i1 MALPY00273 3538 + 163725 167457 407 24 3000
i1 Pc.c001812912.Contig1 1088 - 164231 165319    
i1 Pc.c001405055.Contig1 1460 - 165735 167241    
i1 MALPY01399 8498 - 167606 176770 407 158 6500
i1 PcContig161 1745 - 168194 169966    
i1 Pb.c002007925.Contig1 616 - 168578 169194    
i1 Pb.c000501402.Contig1 2658 - 170057 172778    
i1 Pc.c001606018.Contig1 8566 - 170788 179402    
i1 MALPY03432 378 - 171798 172176   24
i1 MALPY00620 6148 + 176774 182305 407  
i1 Pb.c002005812.Contig1 3686 - 178449 182135  
i1 Pc.c000503139.Contig1 1406 - 179831 181237  
i1 PcContig728 4031 - 181738 185772  
i1 Pb.c000501403.Contig1 5224 + 183770 189399  
i1 MALPY01887 10457 - 184162 196407 1321 158 500
i1 Pc.c000903444.Contig1 757 - 191979 192736    
i1 Pb.c001402406.Contig1 1066 + 191982 193048    
i1 Pb.c000401451.Contig1 3028 - 194116 197825    
i1 MALPY02407 3950 - 197270 201441 1321 158 3000
i1 Pb.c000401169.Contig2 3443 - 198144 201587  
i1 Pc.c001605969.Contig1 2422 + 199282 201707 cRMPc02010 44613 142 157094 201707  
i1 MALPY01308 9441 - 201849 211368 260 172 400
i1 Pb.c000901776.Contig1 10134 + 202452 212664    
i1 MALPY03379 785 - 204349 205134 cRMPc02011 10815 1321 201849 212664   158
i1 MALPY01472 12868 + 213985 226732 260 172 1000
i1 PbContig347 8978 - 217809 226766    
i1 PcContig656 2063 + 222567 224609    
i1 Pc.c001003277.Contig1 3420 - 225870 229293    
i1 Pb.c001502380.Contig1 9448 - 227658 238565    
i1 MALPY03378 1008 - 228173 229184 260 172 300
i1 MALPY03145 1399 - 229111 230507 260 172 350
i1 MALPY02961 2258 - 230617 232955 172 250
i1 Pc.c000602348.Contig1 3858 + 232175 237551  
i1 MALPY02673 4530 - 233904 238677 119 172
i1 Pc.c000603425.Contig1 872 - 234881 235762  
i1 Pc.c001103674.Contig1 3649 + 235274 239139 cRMPc02012 25154 1049 213985 239139  
i1 MALPY01642 5692 - 240188 246109 119
i1 Pc.c001812934.Contig1 522 + 240786 241308  
i1 Pc.c001812935.Contig1 812 + 241566 242390  
i1 Pb.c000401453.Contig1 5570 + 241931 247369  
i1 PcContig537 4779 + 242454 247089  
i1 MALPY00327 12251 - 247118 260254 119
i1 Pc.c001003283.Contig1 2449 + 248210 250849  
i1 Pb.c000301484.Contig1 3211 + 248398 251730  
i1 Pc.c000303386.Contig1 1510 - 253069 254534  
i1 Pb.c001904355.Contig1 4840 - 254369 259416  
i1 Pc.c000502940.Contig1 1589 - 255582 257328  
i1 Pc.c000502938.Contig1 1035 - 257424 258459  
iTK1 MALPY02449 2967 + 260142 263052 119
iman1 MALPY02122 2575 + 261449 264035 cRMPc02013 23847 5531 240188 264035 119
iTK1 MALPY02121 5545 - 269566 275900 cRMPc02014 6334 335 269566 275900 119     cRMPs0201 275900 335 0 275900  
iman1 MALPY02534 2752 + 276235 278692 3
i1 MALPY01244 10812 + 278291 288531 3
i1 Pb.c001502620.Contig1 5610 + 279766 285418  
i1 Pc.c001303494.Contig2 8766 + 281076 290211  
i1 MALPY03239 583 - 282886 283469  
i1 PbContig184 2817 + 287034 289851  
iTK1 MALPY01497 9925 + 288900 300062 3
i1 PbContig262 8753 + 297820 307188  
i1 MALPY01129 9774 - 300819 311420 3 84 600
i1 Pc.c000202196.Contig4 5006 - 300946 306298    
i1 Pc.c001103860.Contig1 2473 - 308149 310631    
i1 Pb.c001702884.Contig1 2330 - 308348 310687    
i1 MALPY02220 4722 - 311055 315792 3 84 3000
i1 PcContig111 7375 - 311157 320165    
i1 Pb.c000201553.Contig1 2530 + 312479 315078    
iTK1 MALPY00774 2072 - 316819 318603 3  
i1 MALPY00402 7116 - 319566 326415 84
i1 Pb.c000901747.Contig1 2676 + 319966 322642
i1 PcContig105 1284 - 320532 321816
i1 Pc.c001708849.Contig1 692 - 322467 323165
i1 Pb.c000801717.Contig1 4074 + 322575 326654
i1 Pc.c000903281.Contig1 2903 - 323517 326420
i1 MALPY03484 715 + 324380 325095
man1 MALPY00382 - BP 29838 + 325522 360971
7 PcContig33 4916 - 327039 332766
7 PbContig174 6132 + 327504 334398
7 Pc.c000703319.Contig1 1104 - 343040 344141
7 PbContig78 7350 - 349342 357471
7 PcContig752 7235 - 349411 357586
7 MALPY03576 1410 + 350894 352336
7 Pc.c001812590.Contig1 1026 + 357713 358757
7 Pb.c001904502.Contig1 1563 + 357889 360055
7 Pc.c001707884.Contig1 1421 + 360243 361520 cRMPc02015 85285 3380 276235 361520       cRMPs0202 85285 3380 276235 361520  
7 PcContig606 6193 - 364900 371662
7 MALPY00694 3060 - 365137 368440
7 Pb.c001803469.Contig1 1967 - 365355 367322
7 MALPY00575 24610 - 366926 392826
7 Pb.c000601622.Contig1 2148 + 370112 372254
7 Pb.c001001746.Contig1 12409 - 375341 388494
7 PcContig196 8037 - 376002 384546
7 Pc.c001304096.Contig1 757 - 381875 382647
7 Pc.c001304357.Contig1 2183 - 387162 389500 cRMPc02016 27926 150 364900 392826       cRMPs0203 27926 150 364900 392826  
7 MALPY00338 5159 + 392976 398138 348 1600
7 Pc.c001505757.Contig1 677 + 395716 396480 cRMPc02017 5162 1357 392976 398138  
7 MALPY00385 7268 - 399495 407285 743 348
7 Pb.c001803870.Contig1 642 - 401598 402243  
7 Pb.c000001701.Contig1 2504 - 403742 406312  
7 Pc.c000102465.Contig1 2883 - 404096 407024 cRMPc02018 7790 867 399495 407285  
7 MALPY00027 9622 + 408152 417568 743
7 Pc.c001812438.Contig1 757 - 411760 412517
7 Pc.c000002042.Contig1 5557 - 413608 418874
7 Pb.c000101614.Contig1 955 - 413991 414946
7 Pb.c000701545.Contig1 2837 - 414951 417627
7 MALPY01240 3996 + 418409 422276 302
7 Pb.c002112309.Contig1 1006 - 420964 421913 cRMPc02019 14124 702 408152 422276  
7 MALPY00406 4832 + 422978 427945 302
7 Pc.c001606530.Contig1 1013 - 424833 425882  
7 Pc.c000702685.Contig1 3143 - 426255 429398  
7 MALPY02984 1121 - 428155 429276 302
7 PbContig300 3499 - 428387 431886  
7 MALPY00344 11744 + 429467 440966 302
7 Pb.c000701523.Contig1 3940 + 436213 439805 cRMPc02020 17988 4300 422978 440966       cRMPs0204 47990 4300 392976 440966  
7 Pb.c000501206.Contig3 9447 + 445266 454651
7 MALPY00838 8112 - 445401 453331 298
7 PcContig517 2320 + 447555 449848  
7 Pc.c000803416.Contig1 3876 + 451052 454931  
7 MALPY00395 51301 - 453955 505650 298
7 Pb.c000301462.Contig1 3182 + 454963 458148  
7 Pc.c000003006.Contig1 2430 + 455704 458134  
7 Pb.c000601255.Contig2 11235 - 465935 477628  
7 PcContig556 7445 + 466793 474299  
7 Pc.c000503503.Contig1 903 + 474480 475347  
7 Pb.c001904789.Contig1 1104 - 477379 478483  
7 Pb.c000701482.Contig1 584 + 477698 478282  
7 PbContig14 37075 - 477886 515013  
7 Pc.c000202745.Contig1 2523 + 481566 483834  
7 Pc.c001813091.Contig1 662 - 489815 490495  
7 PcContig325 3206 - 490924 494112  
7 Pc.c000303027.Contig1 4842 - 494305 499060  
7 Pc.c001404804.Contig1 1432 - 499099 500531  
7 Pc.c000603016.Contig1 1542 - 501471 503001  
7 MALPY03176 183 + 503158 503341  
7 MALPY03403 318 - 503197 503515  
7 Pc.c000002700.Contig1 2292 - 503862 506363  
7 MALPY00850 10395 - 505254 515958 298
7 PcContig743 5634 - 506184 511819
7 MALPY03282 251 - 508526 508786 12 2000
7 MALPY03246 762 - 510274 511033 12 1900
7 PcContig251 2031 + 511205 513426  
7 MALPY02991 660 + 512594 513248 cRMPc02021 70692 11058 445266 515958   12   cRMPs0205 70692 11058 445266 515958  
7 MALPY00426 6008 - 527016 533057
7 Pb.c000701476.Contig1 4412 - 528755 533200
7 PcContig102 6806 + 528971 536613
7 Pb.c001903639.Contig2 1862 + 534196 536052
man7 MALPY00837 - Ins 2743 + 534243 537342
7 PbContig337 9940 + 536773 546667
7 Pc.c000202724.Contig1 2633 + 537313 539928
man7 MALPY01395 9109 - 537681 546790 PbContig337
man7 PbContig6426 1495 - 546612 548107 |
man7 MALPY02541 12895 + 547596 560491 cRMPc02022 33475 527016 560491 cRMPs0206 33475 527016 560491 |
Telomere                                    
Pb Pc Py contig cRMP contig Gap to next cRMP contig cRMP scaffold Gap to next cRMP scaffold
Number 222 Number 22 21 Number 6 5
Average size (bp) 4645 Average size (bp) 23503 2068 Average size (bp) 90211 3845
Minimum size (bp) 183 Minimum size (bp) 2047 79 Minimum size (bp) 27926 150
Maximum size (bp) 51301 Maximum size (bp) 85285 11058 Maximum size (bp) 275900 11058
Total size (bp) 1031181 Total size (bp) 517062 43429 Total size (bp) 541268 19223