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Fig. 8. Plot of the recovery function for a dendrite and a spine with the diffusion coefficient D = 1 and the length of bleached region 2l = 1. As can be seen, the spine-recovery is much faster due to the differences in geometry.

Fig. 9. Calculation of t_1/2 from a recovery curve. The parameter t_1/2 is found in the recovery intensity profile as the time from bleach (t₀) to the time point where the intensity has reached half the final recovery intensity (t_r). The half recovery intensity is found as I_1/2 = I_i + (I_e-I_i)/2, where I_i is the intensity after bleach and I_e is the final recovered intensity.

Movie 1. The video sequence shows a FRAP recording from a dendrite 35 mm long, where the central 10 mm is rapidly bleached. A 30-mW argon laser emitting at 514 nm was used. The first three images in the sequence were recorded pre-bleach to establish a baseline using 2% laser power; then, the central region was bleached with 100% laser power for 2 s, followed by 57 post-bleach recordings at 2% laser power, resulting in a sequence of 5 min in total.