Supplemental Material

This article contains the following supporting material:

• Supplemental Material 2 - Video footage of a DmATP7 mutant first instar larva (DmATP7 - / Y, upper left) next to a rescued DmATP7 mutant larva (DmATP7 - / Y, UAS-DmATP7^wt, lower right). Larvae are of similar age. While the mutant larva is slow and lethargic in its movements and has failed to grow, the rescued larva is as fast and active as wild-type.
• Supplemental Material 1 - Protein sequence alignment showing the high level of conservation between MNK, WND and DmATP7, including complete conservation of all important motifs (highlighted in blue boxes). Identical and similar amino acid residues are shaded dark grey and light grey, respectively. This alignment was generated using CLUSTAL W (Thompson et al., 1994). DmATP7 was identified as the single orthologue of MNK / WND by performing reciprocal BLASTp and psi-BLAST searches (Altschul et al., 1997) and constructing a Neighbor Joining phylogenetic tree (Felsenstein, 1989). This software was accessed using BioManager (ANGIS, http://www.angis.org.au)
  Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402.
  Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.