Supporting information for Lee et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.232308999

 

Table 2. List of selected genes altered in the expression by aging

Identifier

FC, age

%CR effect

Gene

P

 value

pp

Glucose/carbohydrate/energy metabolism

X04591*

1.7

86

Creatine kinase, brain

(Ckb)

0.0016

0.81

AW210370

1.6

8

Homologous to human glucan

(1,4-alpha-), branching enzyme 1

0.0427

0.64

J03928*

1.5

105

Phosphofructokinase, liver, B-type

(Pfk1)

0.0005

0.85

AJ001418*

-8.5

139

Pyruvate dehydrogenase kinase 4

(Pdk4)

0.0020

0.80

D42083*

-5.0

22

Fructose 1,6-bisphosphatase 2

(Fbp2)

0.0013

0.82

AB010742*

-2.6

146

Uncoupling protein 3, mitochondrial

(Ucp3)

0.0038

0.77

Fatty acid metabolism

Fatty acid transport into cytosol and mitochondrial matrix

U15976

-2.0

25

Solute carrier family 27

(fatty acid transporter), member 1 (Slc27a1)

0.0055

0.76

L23108

-1.8

-14

CD36 antigen

(Cd36)

0.0363

0.65

AF017175*

-3.3

46

Carnitine O-palmitoyl transferase I, mitochondrial liver isoform

0.0004

0.86

X85983

-1.6

-15

Carnitine acetyltransferase

(Crat)

0.0211

0.68

AB017112*

-1.5

73

Mitochondrial carnitine/acylcarnitine translocase

(Slc25a20)

0.0011

0.82

Mitochondrial b -oxidation

Y14004*

-5.1

71

Acyl-CoA thioesterase 1, cytosolic

(Cte1)

0.0007

0.84

U69543*

-4.6

89

Lipase, hormone sensitive

(Lipe)

0.0073

0.74

AF006688*

-1.8

38

Acyl-Coenzyme A oxidase 1, palmitoyl

(Acox1)

0.0213

0.68

AW226939*

-1.6

182

Carboxylesterase 3

(Ces3)

0.0154

0.70

AI840013*

-1.6

174

Peroxisomal delta3, delta2-enoyl-coenzyme A isomerase

(Peci)

0.0054

0.76

AF030343

-1.5

13

Enoyl coenzyme A hydratase 1, peroxisomal

(Ech1)

0.0486

0.63

Z14050

-1.5

26

Dodecenoyl-coenzyme A delta isomerase

(Dci)

0.0164

0.70

U89906

4.5

32

Alhpa-methylacyl-CoA racemase

(Amacr)

0.0103

0.72

Neurodegeneration

D29678*

6.3

74

Cyclin-dependent kinase 5

(Cdk5)

0.0051

0.76

AF044672

6.0

-80

Synuclein, alpha

(Snca)

0.0046

0.76

Protein synthesis, modification, and turnover

AJ006584

-2.2

-10

Eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked

(Eif2s3y)

0.0030

0.78

U28656

-1.7

19

Eukaryotic translation initiation factor 4E binding protein 1

(Eif4ebp1)/PHAS-I

0.0002

0.88

AW121930

-1.6

10

Homologous to human eIF4B

0.0122

0.71

AB007139

2.7

129

Proteasome

(prosome, macropain) 28 subunit, 3 (Psme3)

0.0262

0.67

M74227

2.4

7

Peptidylprolyl isomerase C

(Ppic)

0.0062

0.75

AW050342*

1.6

210

Ubiquitin specific protease 23

(Usp23)

0.0085

0.73

AB024303*

1.5

115

Ubiquitin-like 1

(sentrin) activating enzyme E1A (Uble1a)

0.0334

0.65

Extracellular matrix proteins

AF011450*

3.4

134

Procollagen, type XV

(Col15a1)

0.0285

0.66

X57337

2.1

60

Procollagen C-proteinase enhancer protein

(Pcolce)

0.0315

0.66

AF064749*

2.1

189

Type VI collagen alpha 3 subunit

0.0134

0.71

M15832*

1.9

59

Collagen alpha 1

(IV)

0.0007

0.84

D82964

1.7

0

Hyaluronan synthase 1

(Has1)

0.0388

0.64

X60676*

1.5

231

Serine

(or cysteine) proteinase inhibitor, clade H, member 1 (Serpinh1)

0.0048

0.76

Structure, structural modulation, cell–cell interaction, and muscle contraction

AJ010305

15.2

81

Smoothelin

(Smtn)

0.0275

0.67

U82758*

8.1

71

Claudin-5

0.0069

0.74

L48687*

5.9

77

Sodium channel, voltage-gated, type I, beta polypeptide

(Scn1b)

0.0064

0.75

AJ011740

4.2

23

Smarcb1

0.0405

0.64

X13297

2.2

28

Actin, alpha 2, smooth muscle, aorta

(Acta2)

0.0230

0.68

X69902*

1.9

93

Integrin alpha 6

(Itga6)

0.0033

0.78

AF035263

1.7

38

Smarce1

0.0209

0.68

M63801*

1.6

159

Gap junction membrane channel protein alpha 1

(Gja1) (Connexin 43)

0.0073

0.74

X65493*

1.6

162

Intercellular adhesion molecule 2

(Icam2)

0.0273

0.67

AJ131711

1.6

21

Troponin T1, skeletal, slow

(Tnnt1)

0.0005

0.85

Z19543

1.6

67

Calponin 2

(Cnn2)

0.0223

0.68

X69064

1.5

63

Ankyrin 1, erythroid

(Ank1)

0.0338

0.65

AJ000328

-3.3

14

Type 2 desmoglein

0.0086

0.73

Y08027*

-1.6

167

NAD

(P)+ arginine ADP-ribosyltransferase

0.0209

0.68

M91236

-1.6

12

Gap junction membrane channel protein beta 5

(Gjb5) (Connexin 31.1)

0.0131

0.71

AJ010045

-1.6

28

Neuroepithelial cell transforming gene 1

(Net1)

0.0018

0.81

AW121185

-1.5

39

Dynein, cytoplasmic, heavy chain 1

(Dnchc1)

0.0069

0.74

This table lists the genes, of selected classes, that were significantly (P value < 0.05) altered in expression and displayed a fold change greater than 1.5 when comparing 30-month-old control mice with 5-month-old control mice. The last two columns display P values computed by the bootstrap method and their posterior probabilities (pp). %CR effect was computed by ((O – CR)/(O – Y)) ´ 100, where O, CR, and Y are average intensities of 30-month-old control, 30-month-old CR, and 5-month-old control groups, respectively. We note that alterations in gene expression with aging may be associated with alterations in cell populations in the heart.

*Significantly different between CR and control animals.