Presentation of antagonist peptides to naive CD4+ T cells abrogates spatial reorganization of class II MHC peptide complexes on the surface of dendritic cells -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Chmielowski et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.222463499

Fig. 7.
Example of the analysis of expression levels of peptide/MHC complexes tagged with fusion-fluorescent proteins on transduced DCs. Naive CD4⁺ T cells (T) were mixed with A^bIi^- DCs (DC) expressing agonist A^bPCCVA-CFP and null A^bEp-YFP complexes. (A–D) A single 2D optical section in the center of the cell conjugate. (A) A DIC image. (B) A^bEp-YFP only (green). (C) A^bPCCVA-CFP only (blue). (D) A^bPCCVA-YFP and A^bEp-CFP overlay. (B' and C' ) Fluorescence images are color coded according to the scale on the right. (D'' ) Intensity profile is taken from nondeconvolved D'' image along the red line. The histogram shows the fluorescence intensity of the cell membrane. The green solid line corresponds to the intensity of null A^bEp complexes, and the blue dashed line corresponds to the intensity of the agonist A^bPCCVA complexes.