Quantifying signaling-induced reorientation of T cell receptors during immunological synapse formation -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Moss et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.192573999

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Fig. 6.
Time lapse montage showing motion of an individual T cell receptors (TCRs) during T cell activation. (Upper) The fluorescence from a single nanosphere-labeled TCR (red) on a T cell responding to the antigen-presenting cell at the center of the image, overlaid on the differential interference contrast (DIC) image. (Lower) The FURA calcium indicator fluorescence ratio in a false color scale as indicated to the right of the FURA images. Comparing the FURA signal and the DIC/nanosphere overlay, recruitment of the bead to the front of the cell is seen to occur in the first ~2.5 minutes after initial calcium elevation. To view animation, see Movie 2, which is published as supporting information.