Supporting information for Spotts et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.232565699.

We have included four QUICKTIME time-lapse movies that show the time evolution of the CCF-2 R(t)_460/530 for four cortical neurons expressing the KID/KIX-BEAM proteins.

A neuron expressing the KID/KIX-BEAM proteins was identified by detecting fluorescence emission characteristic of the coexpressed DsRed2 red fluorescent protein (Left). The corresponding QUICKTIME time-lapse movie (Right) shows how the CCF-2 R(t)_460/530 for a neuron expressing the KID/KIX-BEAM proteins evolved as a function of time. The neuron presented in A is the same as that presented in Fig. 3 A and B. The pseudocolor scale used for each movie is the same as that used in the manuscript; a rainbow spectrum has been used to represent the CCF-2 R(t)_460/530 values such that neurons containing no hydrolyzed CCF-2 appear at the blue end of the spectrum, whereas neurons in which all of the CCF-2 has been hydrolyzed appear at the red end of the spectrum. The point in time at which the PKA activators forskolin and CPT-cAMP were applied is designated by a red square appearing in a corner of the corresponding R(t)_460/530 image for this time point.