Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Flower number in wild type, rax1-1D/+ and rax1-2 plants. Wild type (Ws-2) and rax1-2plants were grown in SD conditions and wild type (FA4C) and rax1-1D/+ plants were grown in LD conditions, respectively. The total number of flowers produced per plant was counted separately for flowers on the primary stem (includes cauline branches) and on rosette branches. Error bars indicate standard error of the mean.
• Supplemental Figure 2 - RAX1 is ectopically expressed in rax1-1D/+ leaves. RNA was isolated from shoot apices and leaves of wild type (FA4C) or rax1-1D/+ mutant plants, and cDNA synthesized. Quantitative PCR reactions were performed, using primers myb37-4997 and myb37-5684 to amplify RAX1. Numbers below the gel pictures indicate the threshold cycle number (C_t) at which the respective PCR product was scored. Similar quantities of RNA were used for cDNA synthesis for the samples as validated by amplification of eIF4α.
• Supplemental Figure 3 - RAX1 transcripts do not accumulate in rax1-2. RNA was isolated from shoot apices of wild type (Ws-2) or rax1-2 mutant plants, and cDNA synthesized. Quantitative PCR reactions were performed, using primers 5’RT1 and 5’RT2 to amplify RAX1 5’ of the T-DNA insertion site and primers myb37-4997 and myb37-5684 to amplifyRAX1 3’ of the T-DNA insertion, respectively. Numbers below the gel pictures indicate the threshold cycle number (C_t) at which the respective PCR product was scored. (Note that these data were obtained from different amplifications, therefore product amounts appear different on the gel.) Similar quantities of RNA were used for cDNA synthesis for the samples as validated by amplification of eIF4α. nd: not detected.
• Supplemental Figure 4 - Expression of LAS and CUC3 in rax1-1D/+ shoot apices. Shoot apices of wild type (A, C) and rax1-2 (B, D) plants were sectioned and hybridized in situ with anti-sense probes complementary to CUC3(A, B) and LAS(C, D). Arrowheads indicate the CUC3 and LAS transcripts accumulating at the boundary zone between the apical meristem or stem and the leaf primordia. Scale bars: 50 μm.
• Supplemental Figure 5 - The juvenile phase of vegetative development is not altered in rax1 mutants. Leaves were examined for the presence of trichomes on the abaxial (lower) leaf surface. The appearance of trichomes on the abaxial leaf surface is a marker for the adult vegetative phase. The difference between the mean for FA4C and rax1-1D/+ is not significant at the 95% confidence interval. Error bars indicate standard error of the mean.
• Supplemental Figure 6 - GA sprays during vegetative development suppress late-flowering in rax1-1D/+ plants. Plants were grown in long or short days and sprayed with 100μM GA₃twice weekly. Flowering was scored when the inflorescence stem had elongated 1 cm. The number of plants analyzed in each experiment is indicated below and the error bars indicate the standard error of the mean.
• Supplemental Figure 7 - Clustal W alignment of Arabidopsis and tomato Myb genes. The conceptually translated sequences of the Arabidopsis MYB36 (At5g57620), MYB37 (At5g23000), MYB38 (At2g36890), MYB68 (At5g65790), MYB84 (At3g49690), MYB87 (At4g37780), and the Solanum lycopersicum (tomato) Blind DNA binding domains were aligned in Clustal W (DNA Star). Absolutely conserved residues are indicated by a black background, highly conserved residues by a grey background.