The Chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 Activates Multiple Pathogen Resistance Responses -- Yoshioka et al., 10.1105/tpc.105.038786 -- THE PLANT CELL

The Chimeric Arabidopsis CYCLIC NUCLEOTIDE-GATED ION CHANNEL11/12 Activates Multiple Pathogen Resistance Responses
Plant Cell Yoshioka et al. 10.1105/tpc.105.038786

Supplemental Data

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Supplemental Figure 1 - Analysis of T-DNA Insertion Lines and Their F₁ Progeny (A) RT-PCR analyses of the expression of ATCNGC11 or ATCNGC12 in wt Col-0 plants and homozygous T-DNA insertion lines using specific primers (see Methods). 026568: plants homozygous for a T-DNA insertion in ATCNGC11: 092657 and 092622: plants homozygous for a T-DNA insertion in ATCNGC12. Beta-tubulin was used as a loading control. (B) PCR analysis of T-DNA insertions in F₁progeny from the following crosses: 092622 x 026568, 092657 x 026568, or 092657 x 026568. F₁plants were created by reciprocal cross-pollination of three lines. (a) PCR using primers LBb1 and 568RP2 to detect the specific T-DNA insertion in ATCNGC11. (b) PCR using primers LBb1 and 57RP to detect the specific T-DNA insertion in ATCNGC12 (c) PCR using primers LBb1 and 22RP to detect the specific T-DNA insertion in ATCNGC12.
Supplemental Figure 2 - Morphology in wt Ws plants transformed with ATCNGC11and ATCNGC12 driven by the CaMV 35S promoter. Plants were grown on soil and photographed at 4 wpp.