Differential modulation of BMP signaling promotes the elaboration of cerebral cortical GABAergic neurons or oligodendrocytes from a common sonic hedgehog-responsive ventral forebrain progenitor species -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Yung et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.232586699

Fig. 5.
Demonstration of the efficacy of Olig2 and Mash1 gene manipulation paradigms (see Fig. 3). (A) Comparative immunofluorescence micrographs of embryonic day (E) 12.5 fibroblast growth factor (FGF)-responsive cortical progenitor clones after exposure to Ad-GFP (A1, 2) or Ad-O2 (A3, 4) for 6 days in vitro (DIV) demonstrating the presence of similar numbers of GFP-expressing cells (FITC: A1, 3) in both experimental conditions and the selective up-regulation of Olig2 protein expression (tetramethylrhodamine B isothiocyanate: A4 vs. A2) in the Ad-O2 condition. (B and C) Western blot analysis of Mash1 (B) and Olig2 (C) protein expression in E12.5 FGF+Shh-responsive cortical progenitors following propagation for 6 DIV as described (Fig. 3), demonstrating the complete inhibition of Mash1 (B) and Olig2 (C) protein after application of Mash1 and Olig2 antisense oligonucleotides, respectively.