Supporting information for Hofseth et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0237083100
Fig. 8.
(a) Trend analysis of pathology index and levels of inducible NO synthase (iNOS) or P-Ser-15, as indicated. Density of bands shown in b were measured, then compared to the inflammation index. Inflammation index: 0, indicates no significant inflammation; 1, an elevated number of mucosal leukocytes in lamina propria/epithelium; 2, crypt abscesses and/or erosion; 3, crypt abscesses and/or erosion with ulceration. (b) iNOS is induced, p53 accumulates and is modified at serines 15 (phosphorylation) and lysine 382 (acetylation) in ulcerative colitis (UC) but not in normal colon tissue. Controls used were HCT-116 p53-/- (negative immunoprecipitation control) and cells exposed to UV (25 J/m2, 24 h) and Adriamycin (0.34 µM, 4 hr) (positive controls). (c) Controls from Fig. 4c: UC tissue stained with hematoxylin/eosin; UC tissue incubated with antiphosphoserine-15 preabsorbed with the P-Ser-15 blocking peptide or (Inset, the P-Ser-392 peptide, ×100).