Supporting information for Shi et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0136863100
Table 3. Quantitation by real-time RT-PCR of M. tuberculosis mRNAs in the lung of wild-type and IFN-g
–/– mice
M. tuberculosis mRNA |
Mouse strain | Days of infection | |||||||
10 | 12 | 15 | 18 | 21 | 25 | 30 | 50/36* | ||
acr
| WT | 2.92 (1.0) | 3.56 (1.15) | 6.26 (0.17) | 6.68 (0.60) | 7.02 (0.17) | 7.16 (0.31) | 7.03 (0.41) | 6.26 (0.26) |
KO | 2.87 (0.45) | 2.61 (0.36) | 5.78 (0.90) | 6.27 (0.42) | 6.57 (0.30) | 7.49 (0.15) | 8.33 (0.23) | 8.89 (0.27) | |
rv2626c
| WT | 2.32 (0.17) | 3.18 (0.73) | 5.68 (0.34) | 6.22 (0.38) | 6.49 (0.45) | 6.34 (0.40) | 6.24 (0.28) | 5.88 (0.14) |
KO | 2.32 (0.11) | 2.31 (0.61) | 4.08 (0.91) | 5.79 (0.30) | 6.19 (0.14) | 6.99 (0.06) | 7.56 (0.48) | 8.28 (0.34) | |
rv2623
| WT | _ | _ | 4.54 (0.42) | 5.43 (0.59) | 5.58 (0.18) | 5.59 (0.43) | 5.04 (0.62) | 4.84 (0.20) |
KO | _ | _ | 3.66 (1.47) | 4.87 (0.22) | 5.24 (0.25) | 6.34 (0.21) | 7.18 (0.45) | 7.59 (0.53) | |
sodC
| WT | 2.82 (0.35) | 3.47 (0.43) | 4.64 (0.10) | 4.85 (0.27) | 4.64 (0.26) | 4.42 (0.33) | 4.01 (0.20) | 4.13 (0.11) |
KO | 2.67 (0.17) | 3.12 (0.68) | 4.83 (0.55) | 5.20 (0.13) | 5.86 (0.61) | 6.15 (0.29) | 6.57 (0.45) | 7.05 (0.07) | |
sodA
| WT | 3.67 (0.15) | 4.46 (0.33) | 5.39 (0.27) | 5.58 (0.29) | 5.23 (0.06) | 4.90 (0.14) | 4.54 (0.33) | 4.78 (0.06) |
KO | 3.59 (0.46) | 4.35 (0.26) | 5.57 (0.60) | 5.87 (0.09) | 6.25 (0.51) | 6.79 (0.35) | 7.07 (0.32) | 7.75 (0.10) | |
fbpB
| WT | 3.69 (0.37) | 4.66 (0.40) | 5.57 (0.19) | 5.70 (0.28) | 5.46 (0.10) | 5.01 (0.25) | 4.50 (0.23) | 4.71 (0.12) |
KO | 3.81 (0.25) | 3.97 (0.71) | 5.69 (0.67) | 6.03 (0.09) | 6.37 (0.68) | 6.93 (0.21) | 7.19 (0.42) | 7.65 (0.12) | |
Data are shown as log10 of the mean and, in parentheses, the SD of the copy number per lung for each of six M. tuberculosis mRNAs, as specified. For each mRNA, the top row presents results obtained with wild-type (WT) mice and the bottom row shows results for IFN-g –/– knockout (KO) mice. –, below detection.
*This column shows results relative to day 50 of infection in WT mice and to day 36 of infection in KO mice (the latter, immunocompromised animals begin to die around day 36 of infection).