Supporting information for Dziennik et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0237084100
Fig. 8.
Effect of probe type and concentration on overshoot. (A and B) xy confocal images after about 9 min of uptake with a mixed solution of FITC-lysozyme and Cy5-lysozyme with label ratios of 1:40 and 1:1,000, respectively. (A) FITC signal. (B) Cy5 signal. (C and D) xy images after about 9 min of uptake with a mixed solution of FITC-lysozyme and Cy5-lysozyme with label ratios of 1:400 and 1:150, respectively. (C) FITC signal. (D) Cy5 signal. The same particle was used for both cases, using 1 M NaCl to displace the adsorbed protein after the first adsorption experiment. (E and F) Plots of the horizontal intensity profiles (intensity along the solid white line in the image in A) for FITC signals from images A and C (E) and Cy5 signals from images B and D (F). The results show that the overshoot magnitude and position are not affected by the label ratio or the fluorescent species. The high background intensity in the FITC image in C is likely caused by significant scattering of fluorescent light caused by the very high laser power required for the low FITC concentration. (Magnifications: ×220.)