Supporting Figures

Files in this Data Supplement:

Supporting Figure 5
Supporting Figure 6
Supporting Figure 7




Supporting Figure 5

Fig. 5. Nucleotide alignment for all extended family members against the type II germ-line sequence. Replacement mutations are highlighted in green and synonymous mutations in magenta; CDR1, HV2, HV4, and CDR3 are boxed. Residues mentioned in the text as being under negative selection are indicated above the alignment in bold.

Supporting Figure 6

Fig. 6. The nucleotide and amino acid sequence for the type II (germ-line) gene from which the family was derived. The three D regions with all of their possible reading frames are also shown. Solid boxes indicate RGRY motifs and broken boxes WRCY motifs.

Supporting Figure 7

Fig. 7. Wu–Kabat plot of variability for the extended family. Variability was calculated as the number of different amino acids divided by the frequency of the most common amino acid (where frequency is the occurrence of most frequent amino acid divided by the total number of amino acids). Framework regions are shown as gray bars; CDR1, HV2, HV4, and CDR3 are shown as hatched bars; cysteine residues are indicated by black bars. Germ-line-encoded hotspot motifs are indicated by asterisks below the graph.