Mdm4 and Mdm2 cooperate to inhibit p53 activity in proliferating and quiescent cells in vivo -- Francoz et al. 103 (9): 3232 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Francoz et al. 10.1073/pnas.0508476103.

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Supporting Figure 6
Supporting Figure 7

Supporting Figure 6

Fig. 6. Mdm2 and Mdm4 in MEFs. (A and D) Western blot analysis of early passage mouse embryonic fibroblasts (MEFs) either p53^LSL/– (A) or harboring mdm2 or mdm4 floxed alleles (D) infected with adeno-Cre-GFP or adeno-GFP. Vinculin (Vinc.) served as a loading control. Analyses were carried out 24 h after infection. (B and E) Quantitative RT-PCR analysis shows induction of expression of various p53 target genes in cells lacking either Mdm4 or Mdm2 (B) or harboring mdm2 or mdm4 floxed alleles (E) and infected with adeno-Cre. Analyses were carried out 24 h after infection. The data were normalized to the level of expression in control Cre-infected cells and represent the mean (± SD) of three independent experiments. (C and F) Graphical representation showing percentages of cells positive for GFP and BrdUrd from immunofluorescence analysis. The analyses were carried out 24 h after adenoviral infection. The data represent the mean (± SD) of three independent experiments. M2, mdm2; M4, mdm4.

Supporting Figure 7

Fig. 7. Apoptosis in neuronal cells lacking both Mdm2 and Mdm4. (A) Histogram of in situ end labeling (ISEL)-positive cells as a percentage of Nestin-positive cells in the neuroepithelium of embryonic-day-10.5 embryos. Cells were counted in two distinct regions (optic stalk and wall of the midbrain) from three embryos with five sections each. *, P < 0.005. (B) Histogram of ISEL-positive cells as a percentage of b -Tubulin III (b TubIII)-positive cells in the lateral ventricle of embryonic-day-14.5 embryos. Cells were counted from three embryos with five sections each. *, P < 0.005.