Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Expression of HISTONE H4 RNA to monitor S-phase progression by quantitative RT-PCR using Actin-2 (ACT2) as internal control. (A) Relative expression of HISTONE H4 during cell cycle entry after sucrose starvation-induced synchronisation in cell line 35S:CYCD3;1-1 relative to expression observed at T0 after starvation compared to WT (MM2d = black bars; 35S:CYCD3;1-1 = grey bars). (B) Differential expression of HISTONE H4 RNA during exponential growth (exp, d3) in cell line 35S:CYCD3;1-1 presented as relative expression to WT (MM2d, baseline) compared to mutant cell line 35S:S343A presented as relative expression to WT (MM1, baseline) Error bars indicate standard error of the mean; n=4 in A and B.