TABLE 2.
Primers designed for this work
| Region or gene | Name | Sequence (5′ to 3′) | Temp (°C) | Accession no. |
|---|---|---|---|---|
| IS1 from Tn9 | IS1a-F | CTGACGGGGTGGTGCGTAACGGC/ | 65 | NC_002134 |
| IS1a-R | GTAAAACAGCCAGCGCTGGCGCG | |||
| tnpA | tnpA-R100-F | GATCGGCGCGGGGAAGTTCAGGC | 65 | NC_002134 |
| tnpA-R100-R | AGCCGGTTGCAGAGGCCGTAGC | |||
| tnpR | tnpR-R100-F | GAGCGTCAGCGCGAGGGTATTG | —a | AF_071413 |
| istA | istA-F | GACAGCGTTGGCAAGTTAAGTCC | 60 | AF_071413 |
| istA-R | GGCATGCCAGTGGGCATCAAACA | |||
| tniA | tniA-F | GATCCTCCGGCGCACGCTGACCC | 65 | AF_071413 |
| tniA-R | AATCTGGTCGAACGGTTTGGCGG | |||
| urf2 | urf2-F | GGAGATCGAACTGACCGAATCGG | 65 | AF_071413 |
| urf2-R | CAGGAGCTGGCTGCACAACAGC | |||
| merE | merE-F | CGCCCCTGACAAACTGCCGCCC | 60 | AF_071413 |
| merE-R | CGAGCGCGGCAACACCCCAATG | |||
| merD | merD-F | TACGGCCGGTGGCCTGCACCACG | 60 | AF_071413 |
| merD-R | CGCTCGGCTGGCATGGAGGCCAG | |||
| merA | merA-F2 | AAGGCGCACGTGTCACGCTGATC | 70 | AF_071413 |
| merA-R2 | CCGCTGCCTTCTTCAACCACCAG | |||
| merC | merC-F | AGCGTCGTTTCCGCGATGGGCTG | 60 | AF_071413 |
| merC-R | CCGGTCCGCAACGGCGATGCGCC | |||
| merP | merP-F | CTCCCTTGCCCTCGCCGCCGCTG | 65 | AF_071413 |
| merP-R | GGACGGATAGCCGGCGTCTGCGG | |||
| merT | merT-F | GCGCCACGCGAGAAGCGGCCGC | 60 | AF_071413 |
| merT-R | GAAATCCAAGCGCGACCAGGAC | |||
| merR | merR-F | GGGGTCAACGTGGAGACAATCCG | 65 | AF_071413 |
| merR-R | CCTTGAGCTTGTGTTCGGCCAGG | |||
| ybfA | ybfA-F | CGCCCAAGTAAGTATCCAGCTGTG/ | 60 | NC_002525 |
| ybfA-R | CTTGACATCTCATCAATACCACC | |||
| ybfB | ybfB-F | CGATGTGGCTACGAGTCTGATC/ | 55 | NC_002525 |
| ybfB-R | CTTCTCTCTAGATAGCAGGG | |||
| ybgA | ybgA-F | CTATGCACTTTGTGACAAGTTGG/ | 60 | NC_002525 |
| ybgA-R | CTTGAAGTGCAGGCGAGCACG | |||
| tnsA | tnsA-F | GCAGCAGCCTTACAAGACGAGCG | 60 | NC_002525 |
| tnsA-R | GGCTAATACAGAGATCTGCACAC | |||
| tnsB | tnsB-F | TGTAGGTTTGCCAGATGTGTTGC | 60 | NC_002525 |
| tnsB-R | TGACCCCGAGTAATACAAACCCC | |||
| tnsC | tnsC-F | GGCAATTACAGCTTTTACAACGC | 60 | NC_002525 |
| tnsC-R | CTGCGATATCTAGCTGAAGTTGG | |||
| tnsD | tnsD-F | ACAGGGATTGGCTAGTTCACTGG/ | 60 | NC_002525 |
| tnsD-R | CTTTGTGCTTCCTCAGTTATCCG | |||
| tnsE | tnsE-F | GGCGGATCGTGTGATTGAGTTTG/ | 60 | NC_002525 |
| tnsE-R | GCGTACTACCATCACCTGCCTCC |
a
tnpR-R100-F was used together with tnpA-R100-R at an annealing temperature of 65°C.