TABLE 1.

Summary of chemical footprinting data with pleuromutilin derivatives^a

23S rRNA position	No drug	Tiamulin	Valnemulin	SB-264128	Pleuromutilin
A2058	++	++++ E	++++ E	+++ E	+++ E
A2059	+	+++ E	+++ E	++ E	+
G2505	+	− P	− P	− P	− P
U2506	++	− P	− P	+ P	(+) P
U2584	++	+ P	+ P	+++ E	++
U2585	+++	+ P	(+) P	++++ E	++ P

^aE, enhancement; P, protection. The extent of modification with DMS, CMCT, or kethoxal is indicated with + symbols, with (+) and − indicating very weak and no modification, respectively.