See et al. 10.1073/pnas.0508942102

Supporting Information

Files in this Data Supplement:

Supporting Table 1
Supporting Figure 5
Supporting Figure 6





Supporting Figure 5

Fig. 5. Rice (Oryza sativa) Southern blot for Milyang 23 (japonica/indica hybrid) and Gihobyeo (japonica) showing the presence of restriction fragment length polymorphism (RFLP) bands for BF474826 with 80% sequence identity to rice and the absence of RFLP bands for two wheat ESTs, BG263385 and BF201942, with no BLASTn similarity to rice, although a small amount of isotope absorption was observed at the undigested edge of BG263385.





Supporting Figure 6

Fig. 6. Intron and exon prediction in Aegilops sharonensis cosmid sequence presumed orthologous to wheat EST BE497476. The first three panels show alignments with wEST TC264862, TC264868, and TC264853, and the fourth shows FGENESH-predicted ORFs. Exons, introns, and genomic sequence are represented by black regions, open rectangles, and a solid line, respectively.





Table 1. TBLASTX results for ESTs mapped to the long arm of wheat chromosome 4B (4BL) having no sequence homology (NSH) to rice

NSH EST

TC (No. ESTs in TC)

bp

BLASTN

E

 value

TBLASTX

E

 value

BF483117

None

521

Populus balsamifera

4.1

Loblolly pine

0.82

BE591926

TC165287 (2)

553

Antirrhinum majus

2.5

Oryza sativa

0.009

BF483725

TC183026 (2)

560

Hordeum vulgare

1 ´ 10–49

H. vulgare

1e-18

BM140522

TC160397 (3)

704

Secale cereale

2 ´ 10–17

S. cereale

3 ´ 10–5

BE444421

None

539

Vitis vinifera

1.1

Lycopersicon esculentum

0.46

BF201942

TC153135 (9)

1,099

Zea mays

0.001

Vitis

sp../Z. mays

7 ´ 10–28/
8 ´ 10–20

BG263385

None

513

Crocus sativus

0.26

O. sativa

0.044

BE403414

TC190553 (34)

1,047

Puccinellia tenuiflora

2 ´ 10–32

Saccharum officinarum

1 ´ 10–19

BG604822

TC154971 (8)

625

H. vulgare

1 ´ 10–126

H. vulgare

1´ 10–61

BE497476

TC147546 (11)

857

Psoralea tenuiflora

1 ´ 10–27

Saccharum officinarum

5 ´ 10–24

     Shown are NSH wheat EST (wEST), which have no BLASTN sequence homology with rice, the tentative contig (TC) containing the wEST (www.tigr.org/tigr-scripts/tgi/T_index.cgi?species=wheat) with the number of ESTs in the contig shown in parentheses, and the best non-wheat BLASTN and TBLASTX returns from the following databases: dbGSS (genomic sequence surveys), HTGS, RefSeq, and dbEST (www.ncbi.nlm.nih.gov/BLAST).