Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach -- Data Supplement - Supplemental Figures -- Proceedings of the National Academy of Sciences

Supplementary material for Beger et al. (2001) Proc. Natl. Acad. Sci. USA 98 (1), 130-135. (10.1073/pnas.011345898)

Fig. 7.
(A) Schematic diagram of the genomic promoter clone used for reporter constructs (nucleotide position 1-2,894). The promoter includes exon 1 of the NBR2 gene (open box), which is located head-to-head to the BRCA1 gene, and exons 1A, 1B, and 2 of the BRCA1 gene (solid boxes). The hatched box represents a critical region for promoter function containing potential binding sites for the Ets family transcription factors and a CREB binding site (1). The solid eclipse represents the estrogen response element (ERE) (2). Restriction sites: E, EcoRI; P, PstI; S, SapI; X, XbaI. (B) Comparison of endogenous BRCA1 expression, measured by quantitative real-time RT-PCR and normalized to GAPDH message, with EGFP protein expression, measured by FACS as the ratio of BR-EGFP/CMV-EGFP of representative single cell clones. All numbers are given as percentage of SK-BR-3.

References:

1. Suen, T. C. & Goss, P. E. (1999) J. Biol. Chem. 274, 31297-31304.

2. Xu, C. F., Chambers, J. A. & Solomon, E. (1997) J. Biol. Chem. 272, 20994-20997.