Phosphorylation and activation of PINOID by the phospholipid signaling kinase 3-phosphoinositide-dependent protein kinase 1 (PDK1) in Arabidopsis -- Zegzouti et al. 103 (16): 6404 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Zegzouti et al. 10.1073/pnas.0510283103.

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Fig. 7. Response of PINOID (PID) activity to increasing concentrations of MgCl₂ and CaCl₂. (A) PID activity was assayed in the presence of the indicated concentrations of MgCl₂. (B) Response of PID activity to increasing concentrations of CaCl₂ in the presence of 15 mM MgCl₂. For each reaction, PID was allowed to autophosphorylate (Upper) for 45 min before the addition of 3 mg MBP, and transphosphorylation was measured after a 15 min coincubation (Lower).

Fig. 8. PDK1 activation of PINOID (PID) over time. PID–PDK1 complex was incubated in kinase buffer with 10 mCi (1 Ci = 37 GBq)[g-³²P]ATP for the indicated times. The autoradiograph representing phosphorylated PID was exposed for 1 h.