Kinetics and mechanism of electron transfer in intact photosystem II and in the isolated reaction center: Pheophytin is the primary electron acceptor -- Holzwarth et al. 103 (18): 6895 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Holzwarth et al. 10.1073/pnas.0505371103.

Supporting Information

Files in this Data Supplement:

Supporting Text
Supporting Figure 4
Supporting Figure 5
Supporting Table 1
Supporting Figure 6
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Figure 10

Supporting Figure 4

Fig. 4. Results from the application of the kinetic model (A) proposed by Groot et al. (1, 2) to our transient absorption data of isolated D1-D2 RCs (Fig. 5). The resulting SADS are shown in B.

1. van Mourik, F., Groot, M.-L., van Grondelle, R., Dekker, J. P. & van Stokkum, I. H. M. (2004) Phys. Chem. Chem. Phys. 6, 4820–4824.

2. Groot, M.-L., Pawlowicz, N. P., van der Wilderen, L. J. G. W., Breton, J., van Stokkum, I. H. M. & van Grondelle, R. (2005) Proc. Natl. Acad. Sci. USA 102, 13087–13092.

Supporting Figure 5

Fig. 5. Original transient absorption 3D surfaces (before binning; see Supporting Text) for isolated D1-D2 RC excited at 681 nm. (A) The 620- to 740-nm detection range. (B) The 510- to 630-nm detection range (Q_x region). Note that the absorbance scale is reversed (negative is up) for better visibility in the presentation.

Supporting Figure 6

Fig. 6. Original transient absorption kinetics (thin lines) for isolated D1-D2 RCs (cf. Fig. 5) at the indicated detection wavelengths and theoretical curves (thick lines).

Supporting Figure 7

Fig. 7. Original transient absorption 3D surfaces (before binning; see Supporting Text) for intact PSII cores with open RCs excited at 663 nm. (A) The 620- to 740-nm detection range. (B) The 510- to 630-nm detection range (Q_x region). Note that the absorbance scale is reversed (negative is up) for better visibility in the presentation.

Supporting Figure 8

Fig. 8. Original transient absorption kinetics (thin lines) for intact PSII cores (cf. Fig. 7) at the indicated detection wavelengths, and theoretical curves (thick lines).

Supporting Figure 9
Supporting Figure 9 Legend

Supporting Figure 10

Fig. 10. Results of the kinetic compartment analysis for intact PSII cores. (A) Time-dependent populations of the intermediates of intact PSII cores as resulting from the kinetic model in Fig. 3C. The first radical pair RP1 reaches its maximum of 30% relative population at »6 ps after excitation. (B) Amplitude matrix (weighted eigenvectors) for the kinetic model in Fig. 3C. See the Fig. 9 legend for a detailed explanation.

Table 1. Qualitative analysis of lifetime distributions in the LFD maps of the isolated D1-D2 RC

Lifetime range	520 –620 nm range (λ_peak)	620 –670 nm range (λ _peak )	670 –680 nm range (λ_peak)	700 –740 nm range (λ_peak)
100–200 fs	550–600	–	660–690	–
600 fs	–	–	670 w	–
2–4 ps	540-630	–	–	710–740
7–12 ps	(615)	–	(670, 685)	(715)
30–50 ps	540–580, 600–630, broad	640–660, broad	(680)	690–740, broad
100–1,000 ps	510–550, w		640–720, w
³5 ns	520–560, w	640–660	660–700	700–740

Shown are the observed maxima and approximate wavelength ranges (nm) of the lifetime distributions (cf. Fig. 1) in the various detection wavelength ranges. w. weak band.