A plant-based oral vaccine to protect against systemic intoxication by Shiga toxin type 2 -- Wen et al. 103 (18): 7082 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Wen et al. 10.1073/pnas.0510843103.

Supporting Table

Table 2. Oligonucleotides used in this study

Primer	Sequence
Mutagenic primers used to generate stxA_2PO
253F	GTGTATATTGTTTAAATGGGTACTGTGCCTGTTACTGGG
253R	CCCAGTAACAGGCACAGTACCCATTTAAACAATATACAC
532F	CTGATTATTGAGCAAAATAATTTGTCTGTGGCCGGGTTCG
532R	CGAACCCGGCCACAGACAAATTATTTTGCTCAATAATCAG
1119F	GGCGACAGGCCTGTTATAAAAATAAGCAATACATTATGGG
1119R	CCCATAATGTATTGCTTATTTTTATAACAGGCCTGTCGCC
Ligase chain reaction primers used to synthesize stxB_2PO
2B1	GCATGCATCGATCGATGCTAGCTATAGCTAGCATCGATCG
2B2	TCAGAATCATGAAGAAGATGTTCATGGCTGTGCTCTTTGC
2B3	ATTGGCTTCTGTGAATGCATTGGCAGCTGATTGTGCTAAG
2B4	GGTAAGATTGAGTTCTCCAAGTACAATGAGGATGATACAT
2B5	TCACAGTGAAGGTGGATGGGAAGGAGTACTGGACCAGTAG
2B6	GTGGAACCTCCAACCACTCCTCCAAAGTGCTCAATTGACA
2B7	GGAATGACTGTCACAATCAAGTCCAGTACCTGTGAGTCAG
2B8	GCTCTGGATTTGCTGAGGTGCAATTCAACAATGACTAACC
2B9	CGGGATGCGCATAGTATCAGGATCCGATCATGACTGCGTA
2B10	TACGCAGTCATGATCGGATC
2B11	CTGATACTATGCGCATCCCGGGTTAGTCATTGTTGAATTG
2B12	CACCTCAGCAAATCCAGAGCCTGACTCACAGGTACTGGAC
2B13	TTGATTGTGACAGTCATTCCTGTCAATTGAGCACTTTGGA
2B14	GGAGTGGTTGGAGGTTCCACCTACTGGTCCAGTACTCCTT
2B15	CCCATCCACCTTCACTGTGAATGTATCATCCTCATTGTAC
2B16	TTGGAGAACTCAATCTTACCCTTAGCACAATCAGCTGCCA
2B17	ATGCATTCACAGAAGCCAATGCAAAGAGCACAGCCATGAA
2B18	CATCTTCTTCATGATTCTGACGATCGATGCTAGCTATAGC
2B19	TAGCATCGATCGATGCATGC