Antigenic and genetic heterogeneity of Borrelia burgdorferi populations transmitted by ticks -- Data Supplement - Supplemental Figures -- Proceedings of the National Academy of Sciences

Supplementary material for Ohnishi et al. (2001) Proc. Natl. Acad. Sci. USA 98 (2), 670–675. (10.1073/pnas.021546498)

Fig. 8.
Direct dual immunofluorescence staining of Borrelia burgdorferi in unfed tick guts. Gut homogenates of unfed ticks were spotted on a slide, fixed, and stained with fluorescent antibodies. (a and b) The same field double-labeled with a FITC-conjugated polyclonal Borrelia antibody (a) and a Texas Red-conjugated mAb against OspA (b). (c and d) The same field labeled with FITC-conjugated polyclonal Borrelia antibody (c) and a Texas Red-conjugated mAb against OspC (d). Most spirochetes within unfed tick guts stained with the OspA antibody, but none stained with the OspC antibody. (Bars = 20 mm.)