Supplementary material for Carbrey et al. (2001) Proc. Natl. Acad. Sci. USA 98 (3), 1000-1005. (10.1073/pnas.031569898)

Table 1.

 Yeast strains, plasmids, and oligonucleotides

Strain

Genotype

Source/ref.

Genomic database

S288C derivatives

1, 2

S1278b

MATa

J. Boeke

FY86

MATa

, ura3-52, his3D 200, leu2D1

J. Boeke

NRRL-Y-12632

MATa

J. Boeke

GRF5, S. norbensis

MATa

J. Boeke

JC0009

MAT

a, leu2::hisG, trp1D 1, ura3-52, his3::hisG

J. Boeke

JC0012 (MELY11)

MATa

, aqy1D ::loxP-kanMX-loxP

3

JC0015

MATa

, aqy2D ::loxP-kanMX-loxP

 

JC0175

MATa

, aqy1D ::loxP

 

JC0176

MATa

, aqy1D ::loxP, aqy2D::loxP-kanMX-loxP

 

JC0022

MAT

a/a, HIS3/his3::hisG, TRP1/trp1D 1, URA3/ura3-52, LEU2/leu2::hisG

 

JC0102

MATa

, aqy2D ::loxP-kanMX-loxP, ura3-52

 

JC0145

MATa

, aqy2D ::loxP-kanMX-loxP, his3::hisG, ura3-52

This study

JC0023

MAT

a/a, aqy1D ::loxP-kanMX-loxP/aqy1D::loxP-kanMX-loxP,
HIS3/his3::hisG, TRP1/trp1D1, ura3-52/ura3-52

 

JC0123

MAT

a, aqy1D::loxP-kanMX-loxP, aqy2D ::loxP-kanMX-loxP, trp1D1

 

JC0177

MAT

a/a, aqy1D ::loxP-kanMX-loxP/aqy1D::loxP,
aqy2D::loxP-kanMX-loxP/aqy2D::loxP-kanMX-loxP, trp1D 1/TRP1

 

YJM128-1

 

B. Cormack

YJM128-2

 

B. Cormack

YJM309

 

B. Cormack

YJM336

 

B. Cormack

YJM436

 

B. Cormack

Kyokai 7

 

ATCC 26422

S .carlsbergensis

 

S. Michaelis

S. chevalieri

MATa

S. Michaelis

S. diastaticus

 

S. Michaelis

S. exiguus

MATa

S. Michaelis

S. globosus

MATa

S. Michaelis

S. hienipiensis

 

S. Michaelis

S. italicus

 

S. Michaelis

S. norbensis

 

S. Michaelis

S. oleaceus

 

S. Michaelis

S. oleaginosus

 

S. Michaelis

S. oviformis

 

S. Michaelis

Plasmid

Description

Source/ref.

p799201

GRF5 AQY2 ORF and UTR in pCR2.1

 

pGN18201

NRRL-Y-12632 AQY2 ORF and UTR in pCR2.1

 

pFY201

FY86 AQY2 ORF and UTR in pCR2.1

 

p912201

S1278b AQY2 ORF and UTR in pCR2.1

 

p912202

S1278b AQY2 ORF in pCR2.1

 

pX912201

AQY2

 ORF in pXbG-ev1

This study

pXFPVS

AQY2

 ORF with mutations F140V and P141S in pXbG-ev1

 

pXFPAA

AQY2

 ORF with mutations F140A and P141A in pXbG-ev1

 

pXVSFP

AQY1

 ORF with mutations V141F and S142P in pXbG-ev1

 

pGAQY2

S1278b AQY2 ORF in pYES2

 

pYSchev

S. chevalieri AQY2

 ORF in pYES2

 

pXSchev

S. chevalieri AQY2

 ORF in pXG-ev1

 

pX912103

S1278b AQY1 ORF in pXG-ev1

3

pUG6

loxP-KanamycinMX-loxP cassette

4

pSH47

Cre recombinase with GAL1 promoter

4

pXbG-ev1

5' and 3' UTRs of the Xenopus b-globin gene

5

pCR2.1

TA cloning vector

Invitrogen

pYES2

GAL1

 promoter, 2m

Invitrogen

 

Primer

Sequence

ScAQP2-5'

5'-CCCGGATCCACCATGAGTAACGAATCTAACGACCTTG-3'

2ScAQP2-3'

5'-CCCGGATCCCTGAGCAGACTACTCTCAGTCTTCC-3'

5'AQY2

5'-CCCGGATCCCTTGTTCCTGGCTATTACAATTCCTG-3'

3'AQY2

5'-CCCGGATCCCGTAAAAACAGAACTCTGACCAGAAGAG-3'

ScAQP201

5'-TTCCTGAAAATATTCAAAAGCGACACTAGCCTACCTTAGTTATACTAGATTGTACTGAGAGTGCAC-3'

ScAQP202

5'-CGATGGGAGCGTTATGCCAAAAAAGATAAAATTCTGAGCAGACTACTCCTGTGCGGTATTTCACACCG-3'

VSFPa

5'-GGCAACAATCTGCGGAAACCACATAACGAC-3'

VSFPs

5'-GTCGTTATGTGGTTTCCGCAGATTGTTGCC-3'

FPAAa

5'-CAGCAATGATCTGAGCAGCCCACATCACTAC-3'

FPAAs

5'-GTAGTGATGTGGGCTGCTCAGATCATTGCTG-3'

FPVSa

5'-CAGCAATGATCTGAGAAACCCACATCACTAC-3'

FPVSs

5'-GTAGTGATGTGGGTTTCTCAGATCATTGCTG-3'

5'AQY1

5'-GGGAGATCTGTATTTGGTGCTGTCTGTCAATACG-3'

3'AQY1

5'-GGGAGATCTTCAGCTGCTATACGTCAAGGTCGGC-3'

5'KOKanY2

5'-TTCCTGAAAATATTCAAAAGCGACACTAGCCTACCTAAGTTATACTCCAGCTGAAGCTTCGTACGC-3'

3'KOKanY2

5'-CGATGGGAGCGTTATGCCAAAAAAGATAAAATTCTGAGTCAGACTACTCATAGGCCACTAGTGGATCTG-3'

1. Winston, F., Dollard, C. & Ricupero-Hovasse, S. L. (1995) Yeast 11, 53-55.

2. Dujon, B. (1993) Cold Spring Harbor Symp. Quant. Biol. 58, 357-366.

3. Bonhivers, M., Carbrey, J. M., Gould, S. J. & Agre, P. (1998) J. Biol. Chem. 273, 27565-27572.

4. Güldener, U., Heck, S., Fiedler, T., Beinhauer, J. & Hegemann, J. H. (1996) Nucleic Acids Res. 24, 2519-2524.

5. Preston, G. M., Carroll, T. P., Guggino, W. B. & Agre, P. (1992) Science 256, 385-387.