Supporting Figures

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Supporting Figure 6
Supporting Figure 7
Supporting Figure 8

Fig. 6. Quantitative PCR (qPCR) analysis of 7SK knockdown. 7SK sn RNA level knockdown is displayed as a percentage of activity for cells mock-transfected (Control). Cells transfected with unlabeled, antisense (Cy5) labeled, and antisense (Cy5) + sense (Alexa Fluor 488) labeled 7SK siRNA duplexes, all show identical activity within error. See Materials and Methods for details of the assay.

Fig. 7. Down-regulation of HCV replicon levels by NS3 siRNAs. Down-regulation is displayed as a percentage of activity for cells mock-transfected (Control). Cells transfected with the NS3 siRNA unlabeled, and antisense Cy5, sense Alexa Fluor 488, and Cy5 + Alexa Fluor 488 labeled, all show identical activity within error. See Materials and Methods for details of the assay.

Fig. 8. U6 siRNA distribution in live HeLa cells 40 min after transfection. (a-c) Both antisense (red) and sense (green) strands localize in the nucleus (blue). Bright dots visible in the images correspond to siRNA condensed in lipoplexes. (Scale bar, 20 mm.) (d) Overlay of red, green and blue. (e) Two cells (scale bar, 10 mm). (f) Intensity profiles along the selected line (red arrow in d) across two cells confirm that both siRNA strands enter the nucleus.