Figure 3.

ESM1 Is Semidominant.

GC-FID was used to measure the hydrolysis of the endogenous 4-methylsulfinylbutyl glucosinolate into 5MSOP-NIT and 4MSOB-ITC. The structural outcome of endogenous glucosinolate hydrolysis is shown as the ratio of 5MSOP-NIT to the sum of 5MSOP-NIT and 4MSOB-ITC. GC-FID was conducted in the following three lines: CS1945, ESM1^Col/Col; CS1945 × CS1995 F1, ESM1^Col/Ler; and CS1995, ESM1^Ler/Ler. All three lines are homozygous Ler for ESP. Four plants were separately measured per line, and the experiment was independently replicated twice. Lowercase letters above the bars show statistically significant groupings as obtained by t tests within analysis of variance (ANOVA) that combine the two replicate experiments. Error bars indicate se.