Figure 4.

Map-Based Cloning of ESM1.

The genetics methodology used for map-based cloning of the ESM1 QTL is shown.

(A) Approximate map position of the glucosinolate hydrolysis ESM1 QTL on chromosome 3.

(B) Fine-scale mapping of ESM1. Relative marker positions by physical distance are shown, with the number of recombinants per total number of progeny shown below.

(C) BAC contig and genic content for the final recombinant region containing the ESM1 QTL.

(D) Shown for each gene is the maximal difference in transcript accumulation between two accessions when comparing seven Arabidopsis accessions. ANOVA showed that only the At3g14210 and At3g14070 genes had statistical evidence for natural variable transcript accumulation (Kliebenstein et al., 2006).

(E) Semiquantitative RT-PCR of the At3g14210 and At3g14070 candidate genes in Ler and Col-0. RAN and ESP are controls. At3g14225 is a homolog of At3g14210.