Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Genomic Structure and T-DNA Insertion Sites for the AGD1, AGD2, and AGD4 genes. (A) White boxes indicate exons, and each gene is oriented with its 5’ end to the left. AGD1, AGD2, and AGD4 each contain 19 exons and exon sizes were generally conserved. The positions of each T-DNA insertion of the agd1, agd2, and agd4 mutants and that insertion’s corresponding Salk line number are indicated. The positions of oligonucleotides used for real time q-PCR are indicated by black arrows. Arrows connected by a thin broken line indicate oligonucleotides that span exon-exon boundaries. Details about these oligos can be found in the methods. (B) Details of insertion sites for Salk lines used as agd1, agd2, and agd4 mutants.
• Supplemental Figure 2 - Seedling Phenotypes of sfc-9 and agd1 agd2 agd4 sfc-9 quadruple mutants. Seedlings are all 14 days old, grown at 22°C. Quadruple mutants resembled sfc-9, although they were slightly smaller and produced shorter roots. bars = 1 mm
• Supplemental Figure 3 - Additive interaction in the lop1 sfc-1 double mutant. Seedlings, cotyledon vein pattern, and leaf vein patterns. bars = 1 mm
• Supplemental Figure 4 - Root PIN1:GFP Expression and Tracheary Element Differentiation. DIC images were obtained for the same root as shown for the PIN1:GFP expression. In the wild type, differentiated tracheary elements were above the field of view shown in this image. In contrast, tracheary elements differentiate close to the root tip in sfc mutants (shown by the two black arrows). bars = 1 mm.