Rao et al. 10.1073/pnas.0602831103.

Supporting Information

Files in this Data Supplement:

Supporting Data Set 1
Supporting Table 1
Supporting Table 2





Table 1. Oligonucleotides used

miRNA /mRNA

5' primer

3' primer

Annealing temp., ºC/amplicon size, bp

No. of cycles

miR-1-1

taggaactctctcgccagactga

ggcctccttcagagcacagcaa

—/65,333

30

miR-1-2

ggaggatgacttccatactctgaa

actgcacttaaagtacagattggg

65/573

30

miR-133a-1

gcattgccgctgctggtgtagg

ggacagagttactttcttaaggcc

65/562

30

miR-133a-2

atggcccagccagaggacacag

gcagcacctttagcatggaactta

65/351

30

miR-133b

agggctggcaaagctcaatatttg

gcatgttctcgactgcatttccat

65/404

30

Mb(Ex7-10)

cacagggtgcagaaggaggca

ccgttctgactagcagcttgca

65/457

25

Myogenin

caaccaggaggagcgcgatctccg

gctgggtgttagccttatgtgaatgg

65/428

20

Osteocalcin

ttcatgtccaagcaggagggcaa

accgtagatgcgtttgtaggcggt

65/190

25

GAPDH (1)

tgaaggtcggtgtgaacggatttggc

catgtaggccatgaggtccaccac

55/983

22

miR-1 probe

catacttctttacattccatttca

 

50/—

n/a

miR-133 probe

ctggttgaaggggacca

 

50/—

n/a

miR-206 probe

ccacacacttccttacattcca

 

50/—

n/a

U6 probe

ttgcgtgtcatccttgcgcagg

 

50/—

n/a

HsGAPDH (for q-pcr)

ccacatcgctcagacaccat

aaatccgttgactccgacctt

60/51

variable

Hs Myog (for q-pcr)

gtggccttcccagatgaaac

ccggcttggaagacaatctc

60/51

variable

q-pcr, quantitative PCR; n/a, not applicable.

1. Bowen, J. A. & Hunt, J. S. (1999) Biol. Reprod. 60, 428-434.





Table 2. Chip primers used

Region

Forward primer

Reverse primer

Temp, ºC/ Amplicon size, bp

Myog

CCGTCCGTCCAAGACAACCC

CCCCCCTCTAAGCTGTTGC

53/340

Amy2

TCAGTTGTAATTCTCCTTGTACGG

CCTCCCATCTGAAGTATGTGGGTC

61/349

miR133a-2

GGGTCTCTTTCCCTGAGGTC

ATGACAGGTTCCAGGACAGC

60/187

miR206

CCAGGCGCTATTGTACTTCC

TGCCAGTGTCCATTCATCTC

60/190

miR133a-1

TGAAGATAGCACTTGCCTGGT

GGCATCTGCTGACACTGCTA

60/226

miR1-2 peak 1

CCTAGGCTGCTCGAGCTTTA

AGTGTTGGGACCACAGGAAG

60/209

miR1-2 peak 2

ATAGCATGGCAGGCCTTAGA

AGTGGTGCCTAGCCTAATCC

58/158

miR1-1 S1

CCAAGGTGACCTGCAATTCT

CGACACCAACTCATTCCTGA

60/246

miR1-1 S2

CGGTAGCAGGCTCGTAAGG

ATATACCCCGCCAAACATCC

61/220

miR122a

AAGATGACAAGACTCGGGAGA

AGCTTCGGGAACTATGTGGA

59/224