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Video1.mov (3.27MB) Upright twirling in control parasites. The parasite remains fixed to the substrate by its posterior end and pirouettes in a clockwise direction.
Video2.mov (2.77MB) JAS-treated parasite stands on its posterior end and twirls clockwise. Although this parasite spins in the same direction as controls, JAS treatment (2 micromolar in DMSO) results in about 3X faster twirling (see Table I).
Video3.mov (3MB) JAS-treated parasite twirls counterclockwise on its posterior end. JAS treatment causes many parasites to twirl counterclockwise, unlike controls, which only twirl clockwise. Reversed twirling also occurs faster than forward twirling in control cells. JAS-treated (2 micromolar in DMSO).
Video4.mov (1.96MB) JAS-treated parasites repeatedly reverse direction during helical gliding. The parasite in the center of the screen moves both forward towards its apex and backwards towards its posterior. Reversal of direction is only seen after JAS treatment (2 micromolar in DMSO).
Video5.mov (4.87MB) The JAS-treated parasite in the center of the field rolls on the substrate. It frequently reverses direction (vacuole indicates polarity) and no net forward motion results. JAS-treated (2 micromolar in DMSO).