Supporting Figures

Files in this Data Supplement:

Supporting Figure 6
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Figure 10

Fig. 6. Whole body ATP measurement of Da-Gal4/+ and Da-Gal4>UAS-dPink1 RNAi flies 1 week postecolsion, kept continuously at 29°C. Both female and male dPink1 RNAi flies displayed » 70% reduction of ATP level compared with the control flies.

Fig. 7. Male dPink1 RNAi flies showed shorten lifespan.

Fig. 8. Western blot analysis of the expression levels of full-length hPink1 transgenes and hPink1D C transgenes. Each lane represents flies from independent transgenic lines of genotypes as indicated (Da-Gal4>hPink1 or Da-Gal4>hPink1D C), plus control flies (Da/+). Half a fly head and thorax was loaded per lane. Arrows, the position of hPink1D C and full-length hPink1. Asterisk, the position of a nonspecific band, which is very close to the full-length hPink1 band. Flies with matched expression levels of full-length hPink1 and hPink1D C (such as 1F and 1C or 2F and 2C) were selected for subsequent rescuing experiments. b -tubulin serves as a loading control.

Fig. 9. TUNEL staining of IFMs from 4-day-old Da-Gal4/+ (A) and Da-Gal4/UAS-dPink1 RNAi (B) flies kept continuously at 29°C. Left , DAPI staining of nuclei; center, TUNEL staining; right, merged images of DAPI, TUNEL, and Phalloidin-Alexa 498-actin staining.

Fig. 10. Bar graph showing statistical analysis of measurements of brain DA content in control (TH-Gal4/+), TH-Gal4>UAS-dPink1 RNAi, TH-Gal4>UAS-dPink1 RNAi/UAS-hPink1, TH-Gal4>UAS-dPink1 RNAi/UAS-hPink1D C, and TH-Gal4>UAS-dPink1 RNAi/UAS-hParkin animals. Asterisks, P < 0.01 in Student’s t test.