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Supporting Figure 6
Supporting Figure 7

Fig. 6. SOC’s frequency is sensitive to extracellular osmolarity. (A) Reduction of the extracellular solution osmolarity by omitting 25 mM NaCl (in the presence of 1 mM TTX) increases SOC’s frequency (P < 0.01; n = 5 cells). Note that isoosmotic substitution of 25 mM NaCl by choline Cl has no effect. (B) Raising the osmolarity to 360 mOsm (by 16%) with sucrose blocks SOCs.

Fig. 7. Direct block of GABA_A receptors by 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), a putative blocker of volume-regulated anion channels (VRACs). NPPB (100 mM), a blocker of VRACs, reproducibly blocks postsynaptic responses evoked by puff application of GABA (1 mM; 200 ms; two superimposed applications in each case; n = 3 cells).