Prewave pulse and axial wave

The prewave pulse and the beginning of the wave shown in Fig. 3a is shown at 20x real time. The movie represents a 30-sec integration window moving in 2-sec steps. This prewave pulse appears to arise predominantly from the animal pole region, others however appear to emerge nearly simultaneously over large regions of the blastoderm and/or yolk cell membrane.  Higher time resolution studies will be required to determine origination sites and velocity vectors for these pulses.

A wave spreading around the blastoderm margin and then up the primary body axis is shown at 50x real time. The movie represents a 30-sec integration window moving in 5-sec steps. The wave begins on the right side of the marginal zone.