Supporting information for Zhu et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0336132100
Fig. 8.
Partial purification of ovarian progestin receptor and monoclonal antibody screening. (a) Elution profile of solubilized seatrout ovarian membrane extracts on a DEAE column showing peaks of UV absorbance and progestin (17,20β,21-trihydroxy-4-pregnen-3-one) binding activity. Each peak was pooled and concentrated with a 10-kDa molecular mass cutoff filter. Specific progestin binding of each pooled fraction is indicated near each peak. (b) Silver stain of SDS/PAGE gel of the three pooled fractions. Numbers refer to the pooled fractions obtained from DEAE chromatography. (c) Western blot of solubilized ovarian membrane proteins using monoclonal antibody PR10-1. I, solubilized seatrout ovarian plasma membrane proteins; II, seatrout ovarian cytosolic proteins; III, partially purified membrane proteins in fraction 1.