Supporting information for Rustay et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0437273100
Supporting Text
Animals and Husbandry.
Male and female mice from 21 highly inbred strains were obtained from The Jackson Laboratory (Bar Harbor, ME). The strains were selected from the Mouse Phenome Project’s (www.jax.org/phenome) A and B lists based on such criteria as use in gene targeting studies (e.g., 129, FVB, and C57BL/6), widespread usage for other studies, allelic diversity, and wild-strain origin. The 21 strains tested were 129S1/SvImJ, A/J, AKR/J, BALB/cByJ, BTBR T+tf/tf, C3H/HeJ, C57BL/6J, C57L/J, C58/J, CAST/Ei, DBA/2J, FVB/NJ, MOLF/Ei, NOD/LtJ, NZB/BINJ, PERA/Ei, PL/J, SJL/J, SM/J, SPRET/Ei, and SWR/J. Mice were tested between 60 and 120 days of age. Mice were housed at either the Veterinary Medical Unit, Department of Veterans Affairs Medical Center (Portland, OR), or the University of Alberta (Edmonton, AB, Canada). Colony rooms were kept on a 12 h light/12 h dark cycle with lights on at 6 a.m. Room temperature was maintained at 20 ± 2ºC. All procedures were approved by the Institutional Animal Care and Use Committee in accordance with National Institutes of Health guidelines.Drugs.
The 20% ethanol solutions were made by diluting 200-proof ethanol (Pharmco, Brookfield, CT) with 0.9% saline. Intraperitoneal injections were given with volume adjusted according to weight. Control groups were given 0.9% saline.Blood Ethanol Concentrations.
Each mouse was gently restrained and a 20-µl blood sample was taken from the tip of the tail. Blood processing was performed as described (1).1. Boehm, S. L., II, Schafer, G. L., Phillips, T. J., Browman, K. E. & Crabbe, J. C. (2000) Behav. Neurosci. 114, 401–409.