Supplementary material for Reumann et al. (1999) Proc. Natl. Acad. Sci. USA 96(2), 784-789.

   
 pToc75 P.s.      1 .............MRTSVIPNRLTPTLTTHPSRRRNDHITTRTSSLKCHL 37
                                    |   |  |            .      | :   :
 Slr1227         51 LVSPSSELNFPGPTSESEASNYSTLQEAAPSVINALNGENGEISVIPPEI 100
 
 pToc75 P.s.     38 SPSSGDNNDSFNSSLLKTISTTVAVSSAAASAFFLTGSLHSPFPNFSGLN 87
                        ||            :   |      |       .  .  |. . .
 Slr1227        101 EQIDGDRLGQTMEISAGNLDVGVDDLPPMAPVDSAELAQANELPHGNAVA 150
                                                                *
 pToc75 P.s.     88 AAAGGGAGGGGGGSSSSGGGGGGWFNGDEGSFWSRILSPARAIADEPKSE 137
                      |   |        .           |     | ::. | |: :| ..
 Slr1227        151 QVAPRVAQVEQENLIAETKTDN...QTDHVEPQSPLMAQA.AVEEEVEAV 196
 
 pToc75 P.s.    138 DWDSHELPADIT.VLLGRLSGFKKYKISDILFFDRNKKSKVETQDSFLDM 186
                    : :. |    :|         |                  . .
 Slr1227        197 EMEATEETTGVTEETPEETPSFTPDAPPTNTEGTPGPTQTLPSFTPPASP 246
 
 pToc75 P.s.    187 VSLKPGGVYTKAQ.LQKELESLATCGMFEKVDMEGKTNADGSLGLTISFA 235
                     .  |     . . |  |.    |    | .         |
 Slr1227        247 STTTPAPAEEEPRVLVSEVLVTGTTPELELLVYNAIRTQPGRTTTRTQLQ 296
 
 pToc75 P.s.    236 ESMWERADRFRCINVGLMGQSKPVEMDPDMSEKEKIEFFRRQEREYKRRI 285
                    | .          || .     |. .      .    |     |
 Slr1227        297 EDVNAIYATGYFSNVRVAPSDTPLGVRVTFEVQANPVFTGLNIRTVPETA 346
 
 pToc75 P.s.    286 SSARPCLLPTSVHEEIKDMLAEQGRVSARLLQKIRDRVQSWYHEEGYACA 335
                          |    | |   :   .   .. | ||.    :  ||  :||  |
 Slr1227        347 EGKERILPQEVVDETFGEQYGK..ILNLRELQEGIKTINEWYSNQGYDLA 394
 
 pToc75 P.s.    336 QVVNFGNLNT.REVVCEVVEGDITKLSIQYLDKLGNVVEGNTEGPVVQRE 384
                    |||    .    :|   : || :  : :.: |     |:| |   :: ||
 Slr1227        395 QVVGSPQVGADGQVTLVIAEGIVENIQVRFFDSEDEPVQGRTRDFIITRE 444
 
 pToc75 P.s.    385 LPKQLLPGHTFNIEAGKQALRNINSLALFSNIEVNPRPDEMNEGSIIVEI 434
                    :  .| ||  ||    .  |. : || || .: ..  |   .   :|| :
 Slr1227        445 M..RLKPGDVFNRNRAQTDLQRVYSLGLFEDVRLSFNPGS.DPTEVIVNV 491
 
 pToc75 P.s.    435 KLKELEQKSAEVSTEWSIVPGRGGRPTLASLQPGGTITFEHRNLQGLNRS 484
                     . |            ||  | ||  . . |   |||.:: ||| | |..
 Slr1227        492 DVVE........GNTGSIAAG.GGISSSSGL..FGTISYQERNLGGNNQT 530
 
 pToc75 P.s.    485 LTGSVTTSNFLNPQDDLAFKMEYAHPYLDGVDNPRNRTLRVSCFNSRKLS 534
                    :            | :| | . :  |:: |  .|   .   . |  | :|
 Slr1227        531 IGVEAQVG.....QRELLFDVSFTDPWIGG..DPFRTSYTANLFRRRTIS 573
 
 pToc75 P.s.    535 PVFTGGPGVDEVPSIWVDRAGVKANITENFSRQSKFTYGLVMEEIITR.. 582
                     || |        . .     |:  :   | |          :  :.
 Slr1227        574 LVFDGADSSIRTFNGFDSPRVVRTGLGLTFFRPIADDVFAPPDWRLSAGF 623
 
 pToc75 P.s.    583 DESNHICSNGQRVLPNGAISADG...PPTTLSGTGIDRMAFLQANITRDN 629
                       |    |    |   .   .|    | . |  |:| :  |    ..||
 Slr1227        624 GYQNVRIENAAGALSPFSAPLNGFNSQPLSFSDYGVDELFTLSFGASQDN 673
 
 pToc75 P.s.    630 TRFVNGTIVGSRNMFQVDQGLGVGSNFPFFNRHQLTVTKFLQLMSVE.EG 678
                             ||   |  :| : ||.      | . . . :: .  .:  |
 Slr1227        674 RNNALQPTSGSLVRFGAEQTIPVGTGNIMMTRLRGSYSYYIPVNWLDLTG 723
 
 pToc75 P.s.    679 AG...KSPPPVLVLHGHYGGCVGDLPSYDAFTLGGPYSVRGYNMGEIGAA 725
                     |    . |  .  .   |  .|||| |:|| |||  |||||  ||:|
 Slr1227        724 FGLVESTQPQTVAFNVQAGTVLGDLPPYEAFILGGSNSVRGYQEGELGNG 773
 
 pToc75 P.s.    726 RNILELAAEIRIPIKGTHVYA.FAEHGTDLGSSKDVKGNPTVVYRRMGQG 774
                    |.  :  || | ||      | | ::|..|||   | | | :|    | |
 Slr1227        774 RSFFQATAEYRFPIIAAVGGALFVDYGSNLGSQGAVPGFPAIVRGLPGSG 823
 
 pToc75 P.s.    775 SSYGAGMKLGLVRAEYAVD...HNSGTGAVFFRFGERF 809
                      || |.::        :|      |   : |  ||:|
 Slr1227        824 VGYGLGVRIQSPVGPIRIDLGFTGEGESRINFGIGEKF 861

Fig. 5.

 Overall sequence alignment of pea Toc75 and Slr1227 (SynToc75). Pea (Pisum sativum) Toc75 (GenBank accession no. L36858) and Slr1227 (GenBank accession no. D90906) were aligned by using GAP (BLOSUM62, gap weight: 12; length weight: 4) from the Genetics Computer Group (Wisconsin Package version 9.0). Overall sequence identity and similarity are 21% and 28%, respectively. The asterisk indicates the start of mature pea Toc75.