Computational and experimental analysis of DNA shuffling -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Maheshri and Schaffer (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0537968100

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Fig. 4.
Markov reaction model. This diagram represents the algorithm for calculating annealing probabilities for a single collision event. ssDNA, represented by state S, can potentially hybridize to form N unique states, represented by D_1-D_N. Hybridization to form state D_i occurs based on the weighted transition probability X_i/N. If the event does not result in an annealing event, the ssDNA fragments are returned to the unannealed pool. Collision events are then repeated with this pool until no further annealing occurs, at which time one simulated round of DNA shuffling ends.