Sequences in the Agamma -delta intergenic region are not required for stage-specific regulation of the human beta -globin gene locus -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Gaensler et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0634132100

JPEG Image

Fig. 5.
Structural analysis of the ^Aγe-, D2, and D5 YACs. (A) Map of the A20 neo/lys YAC containing the intact human β-globin locus. The functional genes are depicted as black boxes. Vertical arrows indicate the locations of DNase I-hypersensitive sites. The vertical lines labeled "S" indicate SfiI restriction sites, and "Sa" designates a unique SalI restriction site. The black boxes below the linear map designate the positions of probes used in Southern analysis. (B) Southern analysis of EcoRI-restricted WT YAC A85.D10 and ^Aγe–YAC DNA. The human cot 1 probe hybridizes with repeat sequences (Left). A 260-bp locus-specific probe hybridizes with a 3.3-kb EcoRI fragment in the ^Aγe–YAC carrying the 750-bp deletion, and with a 4.1-kb EcoRI fragment in the A85.D10 YAC (Right). (C) Southern analysis of EcoRI-restricted D2 and D5 YAC DNA hybridized with the human cot 1 probe (Left) and a 7.3-kb XbaI fragment specific for the ^Aγ–δ intergenic region (Right). The 7.3-kb probe overlaps three EcoRI fragments, 2.3, 3.1, and 6.95 kb, all of which are present in the WT control (A85 lane). The 3.1-kb fragment is deleted in D2 and D5 lines. The 2.3-kb band is deleted only in the D5 line.