A Cluster of Ring Stage-specific Genes Linked to a Locus Implicated in Cytoadherence in Plasmodium falciparum Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell -- Spielmann et al. 17 (8): 3613 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence in Plasmodium falciparum Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell
Mol. Biol. Cell Spielmann et al. 17: 3613

Supplemental Material

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Table 1
Movie 1 - Rotatable surface projection reconstruction of a REX2-GFP transfected parasite. A series of confocal images was generated from a single infected RBC and reconstructed to form a maximum projection 3-dimensional representation. The spatial animation reveals REX2-GFP associated with Maurer's clefts located at the host cell periphery.
Movie 2 - Animations of images of REX2-GFP associated with immobile Maurer's clefts near the cell periphery as seen in most transfected parasites. Series of images of REX2-GFP transfectants were collected at the time intervals indicated in the movie and are presented as an animation.
Movie 3 - Animations of images of REX2-GFP as observed in some younger stage parasites. In these cells REX2-GFP was associated partly with the PV and partly with mobile structures in the host cell cytoplasm; these may represent nascent Maurer's clefts. Series of images of REX2-GFP transfectants were collected at the time intervals indicated in the movie and are presented as an animation.
Movie 4 - Animation of images of REX3-GFP chimeras. Series of images of REX3-GFP transfectants were collected at the time intervals indicated in the movie and are presented as an animation. The REX3-GFP protein is present throughout the host cell cytoplasm and produces mostly a even fluorescence throughout this compartment. However, bright particles are also observed that are mobile within the host cytoplasm. The animation also shows evidence of much larger structures (bright structures) that are mobile within the cytosol, indicating that some of the REX3-GFP is present as large macromolecular complexes or aggregates within this compartment.